Szutowski M M, Tarczyńska S, Krawczyk J
Zakładu Toksykologii Instytutu Biofarmacji, Akademii Medycznej w Warszawie.
Folia Med Cracov. 1990;31(3):165-74.
Rats were inhaled with C6-C9 petroleum fraction 12 hrs daily for 9 days. The hydrocarbons and their metabolites gas chromatographic profiles from liver, kidney, brain and blood were investigated by means of head space technique. The observed characteristic profile composed of some hydrocarbons and secondary alcohols does not undergo qualitative changes and internal quantitative proportions remain constant in spite of cytochrome P-450 induction. Only total amount of hydrocarbons and their metabolites was varied. The results suggest that C6-C9 petroleum fraction inhalation does not induce new forms of cytochrome P-450 with different catalytic properties, but at the same time substrate binding site is modified to facilitate biotransformation of hydrocarbons and their metabolites.
大鼠每天吸入C6 - C9石油馏分12小时，持续9天。采用顶空技术研究了肝脏、肾脏、大脑和血液中碳氢化合物及其代谢产物的气相色谱图。尽管细胞色素P - 450被诱导，但观察到的由一些碳氢化合物和仲醇组成的特征图谱并未发生定性变化，内部定量比例保持恒定。只是碳氢化合物及其代谢产物的总量有所变化。结果表明，吸入C6 - C9石油馏分不会诱导具有不同催化特性的新形式细胞色素P - 450，但同时底物结合位点会发生改变，以促进碳氢化合物及其代谢产物的生物转化。
