Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Professor Lineu Prestes 1374, 2º andar, sala 247, São Paulo (USP), 05508-900, Brazil.
Mycopathologia. 2011 Apr;171(4):261-6. doi: 10.1007/s11046-010-9375-5. Epub 2010 Oct 24.
The therapeutic efficacy of amphotericin B and voriconazole alone and in combination with one another were evaluated in immunodeficient mice (BALB/c-SCID) infected with a fluconazole-resistant strain of Cryptococcus neoformans var. grubii. The animals were infected intravenously with 3 × 10(5) cells and intraperitoneally treated with amphotericin B (1.5 mg/kg/day) in combination with voriconazole (40 mg/kg/days). Treatment began 1 day after inoculation and continued for 7 and 15 days post-inoculation. The treatments were evaluated by survival curves and yeast quantification (CFUs) in brain and lung tissues. Treatments for 15 days significantly promoted the survival of the animals compared to the control groups. Our results indicated that amphotericin B was effective in assuring longest-term survival of infected animals, but these animals still harbored the highest CFU of C. neoformans in lungs and brain at the end of the experiment. Voriconazole was not as effective alone, but in combination with amphotericin B, it prolonged survival for the second-longest time period and provided the lowest colonization of target organs by the fungus. None of the treatments were effective in complete eradication of the fungus in mice lungs and brain at the end of the experiment.
我们评估了两性霉素 B 和伏立康唑单独及联合应用对氟康唑耐药新型隐球菌格株感染免疫缺陷小鼠（BALB/c-SCID）的治疗效果。动物经静脉注射 3×10(5)个细胞，然后用两性霉素 B（1.5mg/kg/天）联合伏立康唑（40mg/kg/天）进行腹腔内治疗。接种后 1 天开始治疗，持续 7 和 15 天。通过生存曲线和脑组织和肺组织中的酵母定量（CFU）来评估治疗效果。与对照组相比，15 天的治疗显著提高了动物的存活率。我们的结果表明，两性霉素 B 能有效确保感染动物的最长生存时间，但这些动物在实验结束时肺部和脑部的新型隐球菌 CFU 仍然最高。伏立康唑单独应用效果不佳，但与两性霉素 B 联合应用时，能延长第二长的生存时间，并使真菌对靶器官的定植率最低。在实验结束时，没有任何一种治疗方法能有效彻底清除小鼠肺部和脑部的真菌。
