Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
J Ind Microbiol Biotechnol. 2011 Aug;38(8):919-25. doi: 10.1007/s10295-010-0861-z. Epub 2010 Oct 26.
Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.
与许多油脂微生物不同，大肠杆菌细胞中仅维持少量的天然脂质，这阻碍了其用于大量生产脂肪酸的用途。在这项研究中，来自鲍曼不动杆菌的乙酰辅酶 A 羧化酶 (ACC) 在大肠杆菌中表达，以将碳通量重新导向生成丙二酰辅酶 A，这导致细胞内脂质增加了三倍。此外，通过过表达苹果酸酶和向培养基中添加苹果酸来提供高水平的 NADPH，导致细胞内脂质增加了四倍（约 197.74mg/g）。ACC 和苹果酸酶的共表达导致细胞内脂质含量达到 284.56mg/g，与野生型菌株相比增加了 5.6 倍。本研究通过模拟油脂微生物的脂质积累，为提高大肠杆菌中的脂质产量提供了一些有吸引力的策略，这有助于开发原核脂肪酸生产菌。
