Ubels J L, Osgood T B
Department of Ophthalmology, Medical College of Wisconsin, Milwaukee.
J Ocul Pharmacol. 1990 Winter;6(4):321-7. doi: 10.1089/jop.1990.6.321.
When rabbits and humans are treated with orally administered 13-cis retinoic acid, the retinoic acid and a more polar retinoid metabolite appear in tears and lacrimal gland fluid. This study tested the hypothesis that this metabolite was a retinoyl-beta-glucuronide. Lacrimal gland fluid from rabbits treated with a pharmacologic dose of 13-cis retinoic acid was analyzed. Based on chromatographic retention time, absorbance maximum, and degradation to 13-cis retinoic acid by the enzyme beta-glucuronidase, it was shown that this retinoid metabolite is 13-cis retinoyl-beta-glucuronide. The 13-cis retinoyl-beta-glucuronide was also extracted from the lacrimal gland itself. It is concluded that 13-cis retinoyl-beta-glucuronide is a major metabolite of 13-cis retinoic acid in the rabbit and that this retinoid is secreted by the lacrimal gland.
当给兔子和人类口服13 - 顺式视黄酸时，视黄酸和一种极性更强的类视黄醇代谢产物会出现在眼泪和泪腺液中。本研究检验了一种假说，即这种代谢产物是视黄酰 - β - 葡萄糖醛酸苷。对用药理剂量的13 - 顺式视黄酸处理过的兔子的泪腺液进行了分析。基于色谱保留时间、最大吸光度以及该代谢产物被β - 葡萄糖醛酸酶降解为13 - 顺式视黄酸的情况，表明这种类视黄醇代谢产物是13 - 顺式视黄酰 - β - 葡萄糖醛酸苷。13 - 顺式视黄酰 - β - 葡萄糖醛酸苷也从泪腺本身中提取出来了。得出的结论是，13 - 顺式视黄酰 - β - 葡萄糖醛酸苷是兔子体内13 - 顺式视黄酸的主要代谢产物，并且这种类视黄醇是由泪腺分泌的。
