Joe T L, Walker A M
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
J Nutr Sci Vitaminol (Tokyo). 1990 Dec;36(6):609-18. doi: 10.3177/jnsv.36.609.
Previous work from this laboratory has shown mildly elevated dietary leucine to alter the rhythm of prolactin secretion and to interfere with normal estrous cycling in the intact female rat. In this study, we have determined whether these observed effects on prolactin and cycling in turn affected fertility, gestation, mammary gland development or mammary gland morphology during lactation. Groups of control and leucine-supplemented mothers were mated with tested male breeders and a daily record was kept of pup births, pup deaths and general health and development of the pups. The day after weaning, mammary tissue was removed from dams in both groups and prepared for histological examination. In light of the previous results showing estrous cycle interruption and altered prolactin secretion, we report what was a surprising lack of effect of leucine supplementation of fertility, maintenance of gestation or overall development of the mammary glands in this species. Interestingly, however, dietary leucine supplementation was found to markedly after the histological appearance of lactating mammary tissue and to cause a significant increase in the number of litters that contained notably underdeveloped pups.
该实验室之前的研究表明，饮食中亮氨酸轻度升高会改变催乳素的分泌节律，并干扰完整雌性大鼠的正常发情周期。在本研究中，我们确定了这些对催乳素和发情周期的观察效应是否反过来影响了生育能力、妊娠、哺乳期乳腺发育或乳腺形态。将对照组和补充亮氨酸的母鼠与受试雄性种鼠交配，并每日记录幼崽出生、死亡情况以及幼崽的总体健康和发育状况。断奶后第二天，从两组母鼠身上取出乳腺组织，准备进行组织学检查。鉴于之前的结果显示发情周期中断和催乳素分泌改变，我们报告了一个令人惊讶的结果：补充亮氨酸对该物种的生育能力、妊娠维持或乳腺整体发育没有影响。然而，有趣的是，发现饮食中补充亮氨酸会显著改变哺乳期乳腺组织的组织学外观，并导致包含明显发育不全幼崽的窝数显著增加。
