Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 007, India.
J Org Chem. 2010 Nov 19;75(22):7932-5. doi: 10.1021/jo101565e. Epub 2010 Oct 25.
Vorbrüggen coupling of trimethylsilylated 2-N-isobutanoylguanine with peracetylated pentofuranose derivatives generally gives inseparable N-7/N-9 glycosyl mixtures. We have shown that the two isomers can be separated biocatalytically by Novozyme-435-mediated selective deacetylation of the 5'-O-acetyl group of peracetylated N-9 guanine nucleosides.
三甲基硅基保护的 2-N-异丁酰鸟嘌呤与乙酰化的戊呋喃糖衍生物的 Vorbrüggen 偶联通常得到不可分离的 N-7/N-9 糖苷混合物。我们已经表明，通过 Novozyme-435 介导的对乙酰化 N-9 鸟苷核苷的 5'-O-乙酰基的选择性脱乙酰化，可以将两种异构体进行生物催化分离。
