Laboratory of Neurogenetics, Université Montpellier 2, 34095 Montpellier, France.
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19531-6. doi: 10.1073/pnas.1002171107. Epub 2010 Oct 25.
How the peripheral axons of sensory neurons are guided to distant target organs is not well understood. Here we examine this question in the case of the posterior lateral line (PLL) system of zebrafish, where sensory organs are deposited by a migrating primordium. Sensory neurites accompany this primordium during its migration and are thereby guided to their prospective target organs. We show that the inactivation of glial cell line-derived neurotrophic factor (GDNF) signaling leads to defects of innervation and that these defects are due to the inability of sensory axons to track the migrating primordium. GDNF signaling is also used as a guidance cue during axonal regeneration following nerve cut. We conclude that GDNF is a major determinant of directed neuritic growth and of target finding in this system, and we propose that GDNF acts by promoting local neurite outgrowth.
感觉神经元的周围轴突如何被引导到遥远的靶器官尚不清楚。在这里，我们以斑马鱼的后外侧线 (PLL) 系统为例研究这个问题，在这个系统中，感觉器官是由一个迁移的原基沉积的。感觉神经突在原基迁移过程中伴随其迁移，并因此被引导到它们的预期靶器官。我们表明，胶质细胞系衍生的神经营养因子 (GDNF) 信号的失活导致神经支配缺陷，并且这些缺陷是由于感觉轴突无法跟踪迁移的原基。GDNF 信号也被用作神经切断后轴突再生的导向线索。我们得出结论，GDNF 是这个系统中定向神经突生长和靶标发现的主要决定因素，并且我们提出 GDNF 通过促进局部神经突生长而起作用。
