阿片类物质抑制豚鼠脑干切片中的脚桥神经元。

Opiates inhibit pedunculopontine neurones in guinea pig brainstem slices.

作者信息

Serafin M, Khateb A, Mühlethaler M

机构信息

Département de Physiologie, Centre Médical Universitaire, Geneva, Switzerland.

出版信息

Neurosci Lett. 1990 Oct 30;119(1):125-8. doi: 10.1016/0304-3940(90)90772-2.

DOI:10.1016/0304-3940(90)90772-2

PMID:2097575

Abstract

Intracellular recordings were obtained from pedunculopontine neurones in guinea-pig brainstem slices. These cells were characterized by a broad action potential, an A-like conductance and fired spontaneously in a regular manner. These neurones were inhibited by bath-application of both carbachol and serotonine at concentrations of 10(-4) M. Opioid peptides induced a dose-dependent hyperpolarization and a reduction in the spontaneous firing. These latter effects could be blocked by the opiate antagonist naloxone and were direct as they persisted in presence of tetrodotoxine or high magnesium/low calcium-containing salines. They were mediated by an opiate receptor of the mu type since they were obtained with the mu-preferring enkephalin analogues FK 33-824 and DAGO, but neither with the delta nor the kappa analogues such as DPLPE or U-50,488.

摘要

从豚鼠脑干切片中的脚桥核神经元获得细胞内记录。这些细胞的特征是具有宽动作电位、A样电导，并以规则的方式自发放电。在10(-4) M浓度下，向浴槽中施加卡巴胆碱和5-羟色胺均能抑制这些神经元。阿片肽诱导剂量依赖性超极化并减少自发放电。后一种效应可被阿片拮抗剂纳洛酮阻断，并且是直接作用，因为在存在河豚毒素或高镁/低钙盐溶液的情况下这些效应仍然存在。它们是由μ型阿片受体介导的，因为使用偏好μ的脑啡肽类似物FK 33-824和DAGO可观察到这些效应，但使用δ或κ类似物如DPLPE或U-50,488则观察不到。