Khateb A, Serafin M, Mühlethaler M
Département de Physiologie, Centre Médical Universitaire, Geneva, Switzerland.
Neurosci Lett. 1990 May 4;112(2-3):257-62. doi: 10.1016/0304-3940(90)90213-s.
Intracellular recordings were obtained from pedunculopontine tegmental nuclei neurones in guinea pig brainstem slices. These neurones were characterized by the presence of a slow regular firing (around 3 spikes/s), a broad action potential (more than 1 ms) and a transient rectification indicating the presence of an A current. Bath-application of histamine at 10(-4) or 10(-5) M induced a reversible increase in spontaneous firing. In presence of tetrodotoxin (1 microM), the effect of histamine was a reversible membrane depolarization. It was a direct effect as it persisted in presence of a low calcium/high magnesium solution. This excitatory action was presumably mediated by histamine H1 receptors as it could be blocked by the H1 receptor antagonist mepyramine but not by the H2 receptor antagonist cimetidine. A role in arousal is discussed.
在豚鼠脑干切片中，从脚桥被盖核神经元获得细胞内记录。这些神经元的特征是存在缓慢规则放电（约3次/秒）、宽动作电位（超过1毫秒）以及表明存在A电流的瞬时整流。以10(-4)或10(-5) M的浓度浴加组胺可引起自发放电的可逆性增加。在存在河豚毒素（1 microM）的情况下，组胺的作用是使膜发生可逆性去极化。这是一种直接作用，因为在低钙/高镁溶液中它仍然存在。这种兴奋作用可能是由组胺H1受体介导的，因为它可被H1受体拮抗剂美吡拉敏阻断，而不能被H2受体拮抗剂西咪替丁阻断。文中讨论了其在唤醒中的作用。
