外源性铝在培养的大鼠皮层神经元的溶酶体中蓄积。

Exogenous aluminum accumulates in the lysosomes of cultured rat cortical neurons.

作者信息

Schuurmans Stekhoven J H, Renkawek K, Otte-Höller I, Stols A

机构信息

Institute of Pathology, University Hospital Nijmegen, The Netherlands.

出版信息

Neurosci Lett. 1990 Oct 30;119(1):71-4. doi: 10.1016/0304-3940(90)90758-2.

DOI:10.1016/0304-3940(90)90758-2

PMID:2097586

Abstract

In order to study the intracellular localization of aluminum, 0.01% AlCl3 was added to rat cerebral organotypic cultures following 14 days incubation in a standard medium. The cultures were maintained in the aluminum (Al)-containing medium for 1 or 3 days. Subsequently, electron probe X-ray micro-analysis (EPXMA), was used to localize aluminum in the neurons. The Al was found in the cells as early as after 1 day of AlCl3 exposure. The Al was detected exclusively in the neuronal lysosomes, in 66% (1 day exposure) and 97% (3 days) of the measured lysosomes. This localization was confirmed by laser microprobe mass analysis (LAMMA) measurements. Our results demonstrate an Al localization in the neurons, exposed to exogenous Al, different from that in the brains of patients with Alzheimer's disease.

摘要

为了研究铝在细胞内的定位，在标准培养基中孵育14天后，向大鼠脑器官型培养物中添加0.01%的AlCl₃。将培养物在含铝（Al）的培养基中维持1天或3天。随后，使用电子探针X射线微分析（EPXMA）来定位神经元中的铝。早在暴露于AlCl₃ 1天后就在细胞中发现了铝。仅在神经元溶酶体中检测到铝，在所测量的溶酶体中，66%（暴露1天）和97%（暴露3天）含有铝。通过激光微探针质谱分析（LAMMA）测量证实了这种定位。我们的结果表明，暴露于外源性铝的神经元中的铝定位与阿尔茨海默病患者大脑中的不同。