Takeda F, Takeda M, Sato T, Shioda S, Nakai Y
Department of Biochemistry, Showa University School of Medicine, Tokyo, Japan.
Neurosci Lett. 1990 Oct 30;119(1):86-9. doi: 10.1016/0304-3940(90)90762-x.
Content and distribution of Met-enkephalin (Met-ENK)-like immunoreactivity in the hypothalamo-hypophysial system of bovine, rat and frog were examined using specific radioimmunoassay and immunohistochemistry. Ultrastructural localization and co-existence of Met-ENK-, mesotocin (MT)- and vasotocin (VT)-like immunoreactivity in the neural lobe of the frog pituitary was examined by a method combining pre-embedding peroxidase-antiperoxidase immunostaining for Met-ENK with post-embedding immunocolloidal gold staining for MT or VT. The highest concentrations of immunoassayable Met-ENK were present in the neural lobe of the pituitary of the frog. In addition to nerve fibers showing only MT-like or Met-ENK-like immunoreactivity, nerve fibers containing neurosecretory granules showing both MT- and Met-ENK-like immunoreactivities were very rich. But VT-like and Met-ENK-like immunoreactivity was confirmed separately in different axon terminals.
运用特异性放射免疫分析法和免疫组织化学方法，检测了牛、大鼠和青蛙下丘脑 - 垂体系统中脑啡肽（Met-ENK）样免疫反应性的含量及分布。采用包埋前过氧化物酶 - 抗过氧化物酶免疫染色检测青蛙垂体神经叶中Met-ENK样免疫反应性，结合包埋后免疫胶体金染色检测中催产素（MT）或加压催产素（VT）样免疫反应性，以此研究青蛙垂体神经叶中Met-ENK、MT和VT样免疫反应性的超微结构定位及共存情况。可免疫检测的Met-ENK最高浓度存在于青蛙垂体的神经叶中。除了仅显示MT样或Met-ENK样免疫反应性的神经纤维外，含有同时显示MT和Met-ENK样免疫反应性神经分泌颗粒的神经纤维也非常丰富。但VT样和Met-ENK样免疫反应性在不同的轴突终末中分别得到证实。
