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微透析作为一种定量神经肽释放的方法。

Microdialysis as an approach to quantitate the release of neuropeptides.

作者信息

De Mesquita S, Beinfeld M C, Crawley J N

机构信息

Unit on Behavioral Neuropharmacology, National Institute of Mental Health, Bethesda, MD.

出版信息

Prog Neuropsychopharmacol Biol Psychiatry. 1990;14 Suppl:S5-15. doi: 10.1016/0278-5846(90)90083-s.

DOI:10.1016/0278-5846(90)90083-s
PMID:2097673
Abstract
  1. In vivo microdialysis was performed on anesthetized and awake rats to measured release of cholecystokinin from the posterior nucleus accumbens. 2. Basal levels of cholecystokinin were detectable by radioimmunoassay in some, but not all animals. 3. Recovery through the microdialysis probe ranged from 0.1-2.4% for cholecystokinin, suggesting practical limitations to this approach with present technology.
摘要
  1. 对麻醉和清醒的大鼠进行体内微透析,以测量伏隔核后部胆囊收缩素的释放。2. 通过放射免疫测定法在部分而非所有动物中可检测到胆囊收缩素的基础水平。3. 胆囊收缩素通过微透析探针的回收率在0.1%至2.4%之间,这表明现有技术在该方法上存在实际限制。

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1
Microdialysis as an approach to quantitate the release of neuropeptides.微透析作为一种定量神经肽释放的方法。
Prog Neuropsychopharmacol Biol Psychiatry. 1990;14 Suppl:S5-15. doi: 10.1016/0278-5846(90)90083-s.
2
Dual determination of extracellular cholecystokinin and neurotensin fragments in rat forebrain: microdialysis combined with a sequential multiple antigen radioimmunoassay.大鼠前脑中细胞外胆囊收缩素和神经降压素片段的双重测定:微透析结合顺序多重抗原放射免疫测定法
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Microdialysis combined with a sensitive radioimmunoassay. A technique for studying in vivo release of neuropeptides.微透析结合灵敏放射免疫测定法。一种研究神经肽体内释放的技术。
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Cocaine treatment increases extracellular cholecystokinin (CCK) in the nucleus accumbens shell of awake, freely moving rats, an effect that is enhanced in rats that are behaviorally sensitized to cocaine.可卡因治疗可增加清醒、自由活动大鼠伏隔核壳中的细胞外胆囊收缩素(CCK),在对可卡因产生行为敏化的大鼠中,这种作用会增强。
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The in vivo microdialysis recovery of dopamine is altered independently of basal level by 6-hydroxydopamine lesions to the nucleus accumbens.通过对伏隔核进行6-羟基多巴胺损伤,多巴胺的体内微透析回收率不受基础水平的影响而发生改变。
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Use of microdialysis in neuroendocrinology.微透析技术在神经内分泌学中的应用。
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[W-probe for microdialysis method in freely moving rat brain].
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