Lindefors N, Brodin E, Ungerstedt U
J Pharmacol Methods. 1987 Jul;17(4):305-12. doi: 10.1016/0160-5402(87)90044-1.
Microdialysis in combination with radioimmunoassay was used to study in vivo release of neuropeptides in the rat brain. Microdialysis is a novel brain perfusion technique, by which molecules in the extracellular space may be monitored. This study describes the application to neuropeptide research. A radioimmunoassay procedure was adapted so that substance P- and neurokinin A-like immunoreactivity could be measured in samples collected during in vivo release experiments. The detection limits of substance P and neurokinin A were below 0.1 fmol/100-microliters sample and the IC50 approximately 1 fmol/sample for both peptides. Potassium, introduced via the microdialysis probe, was found to induce a concentration-dependent increase of the extracellular concentration of neurokinin A-like immunoreactivity.
采用微透析结合放射免疫分析法研究大鼠脑内神经肽的体内释放。微透析是一种新型的脑灌注技术，通过该技术可监测细胞外空间中的分子。本研究描述了其在神经肽研究中的应用。对放射免疫分析程序进行了调整，以便能够在体内释放实验期间收集的样本中测量P物质和神经激肽A样免疫反应性。P物质和神经激肽A的检测限低于0.1 fmol/100微升样本，两种肽的半数抑制浓度（IC50）约为1 fmol/样本。经微透析探针注入的钾离子可诱导神经激肽A样免疫反应性细胞外浓度呈浓度依赖性增加。
