艰难梭菌感染的 aged gnotobiotic C57BL/6 小鼠和 BI/NAP1 株的鼠模型。
Murine model of Clostridium difficile infection with aged gnotobiotic C57BL/6 mice and a BI/NAP1 strain.
机构信息
Center For Global Health, Division of Infectious Diseases and International Health, Department of Internal Medicine, University of Virginia, Charlottesville, VA 22908, USA.
出版信息
J Infect Dis. 2010 Dec 1;202(11):1708-12. doi: 10.1086/657086. Epub 2010 Oct 26.
Abstract
The increased incidence and severity of Clostridium difficile infection (CDI) in older adults (age, ≥65 years) corresponds with the emergence of the BI/NAP1 strain, making elucidation of the host immune response extremely important. We therefore infected germ-free C57BL/6 mice aged 7-14 months with a BI/NAP1 strain and monitored the mice for response. Infected mice were moribund 48-72 h after infection and developed gross and histological cecitis and colitis and elevated concentrations of keratinocyte chemoattractant, interleukin 1β, monocyte chemotactic protein 1, and granulocyte colony-stimulating factor and decreased levels of interferon γ, interleukin 12 p40, interleukin 12 p70, and interleukin 10 compared with controls. We conclude that aged, germ-free C57BL/6 mice are susceptible to fulminant CDI from a BI/NAP1 strain and represent a novel model to further elucidate the host immune response to acute CDI.
摘要
老年（年龄≥65 岁）人群中艰难梭菌感染（CDI）的发生率和严重程度增加与 BI/NAP1 株的出现相对应，因此阐明宿主免疫反应极其重要。我们用 BI/NAP1 株感染了 7-14 月龄的无菌 C57BL/6 小鼠，并监测了小鼠的反应。感染后 48-72 小时，感染小鼠濒死，并出现明显的盲肠结肠炎和结肠炎，角蛋白细胞趋化因子、白细胞介素 1β、单核细胞趋化蛋白 1 和粒细胞集落刺激因子浓度升高，干扰素 γ、白细胞介素 12 p40、白细胞介素 12 p70 和白细胞介素 10 水平降低与对照组相比。我们得出结论，老年无菌 C57BL/6 小鼠易发生由 BI/NAP1 株引起的暴发性 CDI，代表了进一步阐明宿主对急性 CDI 免疫反应的新型模型。
相似文献
1
Murine model of Clostridium difficile infection with aged gnotobiotic C57BL/6 mice and a BI/NAP1 strain.艰难梭菌感染的 aged gnotobiotic C57BL/6 小鼠和 BI/NAP1 株的鼠模型。
J Infect Dis. 2010 Dec 1;202(11):1708-12. doi: 10.1086/657086. Epub 2010 Oct 26.
2
Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice.艰难梭菌感染会加重白细胞介素10缺陷小鼠的结肠炎。
World J Gastroenterol. 2014 Dec 7;20(45):17084-91. doi: 10.3748/wjg.v20.i45.17084.
3
Piglet models of acute or chronic Clostridium difficile illness.急性或慢性艰难梭菌病的小猪模型。
J Infect Dis. 2010 Feb 1;201(3):428-34. doi: 10.1086/649799.
4
Immune responses to Clostridium difficile infection.对艰难梭菌感染的免疫反应。
Trends Mol Med. 2012 Nov;18(11):658-66. doi: 10.1016/j.molmed.2012.09.005. Epub 2012 Oct 16.
5
NAP1 strain type predicts outcomes from Clostridium difficile infection.NAP1菌株类型可预测艰难梭菌感染的结果。
Clin Infect Dis. 2014 May;58(10):1394-400. doi: 10.1093/cid/ciu125. Epub 2014 Mar 5.
6
Cefoperazone-treated mice as an experimental platform to assess differential virulence of Clostridium difficile strains.以头孢哌酮处理的小鼠作为实验平台来评估艰难梭菌菌株的差异毒力。
Gut Microbes. 2011 Nov-Dec;2(6):326-34. doi: 10.4161/gmic.19142. Epub 2011 Nov 1.
7
Intrarectal instillation of Clostridium difficile toxin A triggers colonic inflammation and tissue damage: development of a novel and efficient mouse model of Clostridium difficile toxin exposure.经直肠滴注艰难梭菌毒素 A 可引发结肠炎症和组织损伤：艰难梭菌毒素暴露的新型且高效的小鼠模型的建立。
Infect Immun. 2012 Dec;80(12):4474-84. doi: 10.1128/IAI.00933-12. Epub 2012 Oct 8.
8
Modeling the role of peroxisome proliferator-activated receptor γ and microRNA-146 in mucosal immune responses to Clostridium difficile.模拟过氧化物酶体增殖物激活受体 γ 和 microRNA-146 在艰难梭菌黏膜免疫反应中的作用。
PLoS One. 2012;7(10):e47525. doi: 10.1371/journal.pone.0047525. Epub 2012 Oct 11.
9
Fluoroquinolone and Macrolide Exposure Predict Clostridium difficile Infection with the Highly Fluoroquinolone- and Macrolide-Resistant Epidemic C. difficile Strain BI/NAP1/027.氟喹诺酮类和大环内酯类药物的暴露可预测艰难梭菌感染，其感染菌株为对氟喹诺酮类和大环内酯类高度耐药的流行菌株BI/NAP1/027。
Antimicrob Agents Chemother. 2015 Nov 2;60(1):418-23. doi: 10.1128/AAC.01820-15. Print 2016 Jan.
10
IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection.IL-33 驱动 2 型固有淋巴细胞介导的艰难梭菌感染保护作用。
Nat Commun. 2019 Jun 20;10(1):2712. doi: 10.1038/s41467-019-10733-9.
引用本文的文献
1
Reviewing the Mouse Model: Insights into Infection Mechanisms.回顾小鼠模型：对感染机制的见解。
Microorganisms. 2024 Jan 27;12(2):273. doi: 10.3390/microorganisms12020273.
2
CDBN-YGXZ, a Novel Small-Molecule Drug, Shows Efficacy against Clostridioides difficile Infection and Recurrence in Mouse and Hamster Infection Models.CDBN-YGXZ，一种新型小分子药物，在艰难梭菌感染和复发的小鼠和仓鼠感染模型中显示出疗效。
Antimicrob Agents Chemother. 2023 May 17;67(5):e0170422. doi: 10.1128/aac.01704-22. Epub 2023 Apr 13.
3
The impact of dietary fibers on infection in a mouse model.膳食纤维对小鼠模型中感染的影响。
Front Cell Infect Microbiol. 2022 Nov 9;12:1028267. doi: 10.3389/fcimb.2022.1028267. eCollection 2022.
4
Ameliorates Infection in Mice by Modulating the Intestinal Microbiome and Metabolites.通过调节肠道微生物群和代谢产物改善小鼠感染情况。
Front Microbiol. 2022 May 18;13:841920. doi: 10.3389/fmicb.2022.841920. eCollection 2022.
5
Teaching old mice new tricks: the utility of aged mouse models of C. difficile infection to study pathogenesis and rejuvenate immune response.教老老鼠新把戏：艰难梭菌感染的老年小鼠模型在研究发病机制和恢复免疫反应方面的应用。
Gut Microbes. 2021 Jan-Dec;13(1):1966255. doi: 10.1080/19490976.2021.1966255.
6
Longitudinal Changes in Diet Cause Repeatable and Largely Reversible Shifts in Gut Microbial Communities of Laboratory Mice and Are Observed across Segments of the Entire Intestinal Tract.饮食的纵向变化会引起实验小鼠肠道微生物群落的可重复且主要可逆转的变化，并在整个肠道的各个部位都能观察到。
Int J Mol Sci. 2021 Jun 1;22(11):5981. doi: 10.3390/ijms22115981.
7
Modeling Enteropathy or Diarrhea with the Top Bacterial and Protozoal Pathogens: Differential Determinants of Outcomes.利用主要细菌和原生动物病原体建立肠病或腹泻模型：结局的差异决定因素
ACS Infect Dis. 2021 May 14;7(5):1020-1031. doi: 10.1021/acsinfecdis.0c00831. Epub 2021 Apr 26.
8
Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against .定植因子CD0873，一种有吸引力的抗……口服疫苗候选物
Microorganisms. 2021 Feb 2;9(2):306. doi: 10.3390/microorganisms9020306.
9
In vivo animal models confirm an increased virulence potential and pathogenicity of the NAP1/RT027/ST01 genotype within the MLST Clade 2.体内动物模型证实，在多位点序列分型进化枝2内，NAP1/RT027/ST01基因型的毒力潜力和致病性有所增加。
Gut Pathog. 2020 Sep 22;12:45. doi: 10.1186/s13099-020-00383-4. eCollection 2020.
10
The glucosyltransferase activity of C. difficile Toxin B is required for disease pathogenesis.艰难梭菌毒素 B 的葡糖基转移酶活性是疾病发病机制所必需的。
PLoS Pathog. 2020 Sep 22;16(9):e1008852. doi: 10.1371/journal.ppat.1008852. eCollection 2020 Sep.
本文引用的文献
1
Toxin B is essential for virulence of Clostridium difficile.毒素B对于艰难梭菌的毒力至关重要。
Nature. 2009 Apr 30;458(7242):1176-9. doi: 10.1038/nature07822. Epub 2009 Mar 1.
2
A mouse model of Clostridium difficile-associated disease.艰难梭菌相关性疾病的小鼠模型
Gastroenterology. 2008 Dec;135(6):1984-92. doi: 10.1053/j.gastro.2008.09.002. Epub 2008 Sep 10.
3
Increase in adult Clostridium difficile-related hospitalizations and case-fatality rate, United States, 2000-2005.2000 - 2005年美国成人艰难梭菌相关住院率及病死率上升情况
Emerg Infect Dis. 2008 Jun;14(6):929-31. doi: 10.3201/eid1406.071447.
4
IL-12 and type-I IFN synergize for IFN-gamma production by CD4 T cells, whereas neither are required for IFN-gamma production by CD8 T cells after Listeria monocytogenes infection.白细胞介素-12和I型干扰素协同作用，促使CD4 T细胞产生γ干扰素，而在单核细胞增多性李斯特菌感染后，CD8 T细胞产生γ干扰素则既不需要白细胞介素-12也不需要I型干扰素。
J Immunol. 2007 Apr 1;178(7):4498-505. doi: 10.4049/jimmunol.178.7.4498.
5
Surface layer proteins from Clostridium difficile induce inflammatory and regulatory cytokines in human monocytes and dendritic cells.艰难梭菌的表层蛋白可在人单核细胞和树突状细胞中诱导炎性细胞因子和调节性细胞因子。
Microbes Infect. 2006 Sep;8(11):2640-6. doi: 10.1016/j.micinf.2006.07.009. Epub 2006 Aug 8.
6
An epidemic, toxin gene-variant strain of Clostridium difficile.一种艰难梭菌的流行、毒素基因变异菌株。
N Engl J Med. 2005 Dec 8;353(23):2433-41. doi: 10.1056/NEJMoa051590. Epub 2005 Dec 1.
7
Variable phenotypes of enterocolitis in interleukin 10-deficient mice monoassociated with two different commensal bacteria.与两种不同共生菌单关联的白细胞介素10缺陷小鼠中肠炎的可变表型
Gastroenterology. 2005 Apr;128(4):891-906. doi: 10.1053/j.gastro.2005.02.009.
8
Differential effects of varying concentrations of clostridium difficile toxin A on epithelial barrier function and expression of cytokines.不同浓度艰难梭菌毒素A对上皮屏障功能及细胞因子表达的差异影响
J Infect Dis. 2004 Jun 1;189(11):2110-9. doi: 10.1086/386287. Epub 2004 Apr 30.
9
Essential involvement of IFN-gamma in Clostridium difficile toxin A-induced enteritis.γ-干扰素在艰难梭菌毒素A诱导的肠炎中起重要作用。
J Immunol. 2004 Mar 1;172(5):3018-25. doi: 10.4049/jimmunol.172.5.3018.
10
Structure of the intestinal flora responsible for development of the gut immune system in a rodent model.在啮齿动物模型中负责肠道免疫系统发育的肠道菌群结构。
Microbes Infect. 2000 Sep;2(11):1343-51. doi: 10.1016/s1286-4579(00)01288-0.
Zotero 插件