Center For Global Health, Division of Infectious Diseases and International Health, Department of Internal Medicine, University of Virginia, Charlottesville, VA 22908, USA.
J Infect Dis. 2010 Dec 1;202(11):1708-12. doi: 10.1086/657086. Epub 2010 Oct 26.
The increased incidence and severity of Clostridium difficile infection (CDI) in older adults (age, ≥65 years) corresponds with the emergence of the BI/NAP1 strain, making elucidation of the host immune response extremely important. We therefore infected germ-free C57BL/6 mice aged 7-14 months with a BI/NAP1 strain and monitored the mice for response. Infected mice were moribund 48-72 h after infection and developed gross and histological cecitis and colitis and elevated concentrations of keratinocyte chemoattractant, interleukin 1β, monocyte chemotactic protein 1, and granulocyte colony-stimulating factor and decreased levels of interferon γ, interleukin 12 p40, interleukin 12 p70, and interleukin 10 compared with controls. We conclude that aged, germ-free C57BL/6 mice are susceptible to fulminant CDI from a BI/NAP1 strain and represent a novel model to further elucidate the host immune response to acute CDI.
老年（年龄≥65 岁）人群中艰难梭菌感染（CDI）的发生率和严重程度增加与 BI/NAP1 株的出现相对应，因此阐明宿主免疫反应极其重要。我们用 BI/NAP1 株感染了 7-14 月龄的无菌 C57BL/6 小鼠，并监测了小鼠的反应。感染后 48-72 小时，感染小鼠濒死，并出现明显的盲肠结肠炎和结肠炎，角蛋白细胞趋化因子、白细胞介素 1β、单核细胞趋化蛋白 1 和粒细胞集落刺激因子浓度升高，干扰素 γ、白细胞介素 12 p40、白细胞介素 12 p70 和白细胞介素 10 水平降低与对照组相比。我们得出结论，老年无菌 C57BL/6 小鼠易发生由 BI/NAP1 株引起的暴发性 CDI，代表了进一步阐明宿主对急性 CDI 免疫反应的新型模型。
