Liu Hong-Zhen, Zhong Jing-Quan, Li Jing-Sha, Su Guo-Ying, Wang Jing, Zhang Yun
Department of Cardiology, Qilu Hospital Affiliated to Shandong University, Jinan 250012, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Oct;22(10):595-8.
To observe changes in connexin 43 (Cx43) after ventricular fibrillation (VF) and the effects of rotigaptide (ZP123) on Cx43.
Thirty domestic pigs were randomly assigned to three groups (10 in each group): sham group, model group and ZP123 group. VF was induced by an 80 V AC transthoracic shock for 5 seconds with the use of subcutaneous needles. Before the induction of VF, animals in ZP123 group were administered with ZP123 (1 μg/kg bolus+10 μg×kg(-1)×h(-1) dissolved in 50 ml normal saline and pumped for 15 minutes ). Those in model group received 50 ml normal saline pumped for 15 minutes. For pigs in sham group VF was not induced and no fluid was given. After 8 minutes of VF, animals were euthanized and myocardial tissues were harvested along the long axis of each left ventricular free wall. Immunofluorescence combined with laser scanning confocal microscope was used to detect the distribution of Cx43. Western blotting was used for quantitative determination of Cx43 protein expression.
Immunofluorescence signals for Cx43 in sham group were strong and regularly distributed. In model group, Cx43 signals were weak and distributed in heterogeneity, while in ZP123 group, Cx43 signals were enhanced and their distribution were much more orderly. Compared with sham group, the percentage area and the optical densities (A value) of Cx43 fluorescence signals and Cx43 protein expression were significantly decreased in model group [the percentage area: (0.64±0.36)% vs.(1.27±0.19)%, A value: 15 201± 2 613 vs. 30 634±4 975, Cx43 protein expression: 0.72±0.08 vs. 0.97±0.07, all P<0.05]. The level of Cx43 expression in ZP 123 group [the percentage area (0.96±0.16)%, A value 22 100±4 404, Cx43 protein expression 0.82±0.04] was much higher than model group (all P<0.05).
During VF, down-regulation of myocardial Cx43 expression occurred, which could be attenuated by administration of ZP123.
观察心室颤动（VF）后连接蛋白43（Cx43）的变化以及罗替加滨（ZP123）对Cx43的影响。
30只家猪随机分为三组（每组10只）：假手术组、模型组和ZP123组。使用皮下针经胸80V交流电电击5秒诱导VF。在诱导VF前，ZP123组动物给予ZP123（1μg/kg静脉推注+10μg×kg⁻¹×h⁻¹溶于50ml生理盐水中并泵注15分钟）。模型组动物接受50ml生理盐水泵注15分钟。假手术组猪不诱导VF且不给任何液体。VF持续8分钟后，处死动物并沿左心室游离壁长轴取心肌组织。采用免疫荧光结合激光扫描共聚焦显微镜检测Cx43的分布。蛋白质印迹法用于定量测定Cx43蛋白表达。
假手术组Cx43免疫荧光信号强且分布规则。模型组Cx43信号弱且分布不均，而ZP123组Cx43信号增强且分布更有序。与假手术组相比，模型组Cx43荧光信号的面积百分比和光密度（A值）以及Cx43蛋白表达均显著降低[面积百分比：（0.64±0.36）%对（1.27±0.19）%，A值：15201±2613对30634±4975，Cx43蛋白表达：0.72±0.08对0.97±0.07，均P<0.05]。ZP123组Cx43表达水平[面积百分比（0.96±0.16）%，A值22100±4404，Cx43蛋白表达0.82±0.04]显著高于模型组（均P<0.05）。
VF期间，心肌Cx43表达下调，而给予ZP123可使其减轻。
