Department of Physics, Chemistry and Biology (IFM), Linköping University, S-581 83 Linköping, Sweden.
Acta Biomater. 2011 Mar;7(3):1369-78. doi: 10.1016/j.actbio.2010.10.024. Epub 2010 Oct 25.
Thin films of amorphous carbon and amorphous, graphitic and fullerene-like carbon nitride were deposited by reactive magnetron sputtering and optically characterized with spectroscopic ellipsometry. Complementary studies using scanning electron microscopy and atomic force microscopy were performed. The films were exposed to human serum albumin (HSA) and the adsorption was monitored in situ using dynamic ellipsometry. From the ellipsometric data the adsorbed amount of proteins was quantified in terms of surface mass density using de Feijter's model. The results indicate larger adsorption of proteins onto the amorphous films compared to the films with a more textured structure. Complementary studies with 125I-labeled HSA showed an apparent protein adsorption up to six times larger compared to the ellipsometry measurement. In addition, the four types of films were incubated in blood plasma followed by exposure to anti-fibrinogen, anti-HMWK or anti-C3c, revealing the materials' response to complement and contact activation. The amorphous and graphitic carbon nitride exhibit rather high immune activity compared to a titanium reference, whereas the amorphous carbon and the fullerene-like CNx show less immune complement deposition. Compared to the reference, all films exhibit indications of a stronger ability to initiate the intrinsic pathway of coagulation. Finally, the surfaces' bone-bonding ability was investigated by examination of their ability to form calcium phosphate crystals in a simulated body fluid, with a-CNx depositing most calcium phosphate after 21 days of incubation.
采用反应磁控溅射法制备了非晶碳和非晶、石墨和类富勒烯碳氮化物薄膜，并采用光谱椭圆偏振法进行了光学特性表征。同时还进行了扫描电子显微镜和原子力显微镜的补充研究。将这些薄膜暴露于人血清白蛋白（HSA）中，并使用动态椭圆偏振法原位监测吸附情况。根据椭圆偏振数据，使用 de Feijter 模型从表面质量密度方面定量了蛋白质的吸附量。结果表明，与具有更具织构结构的薄膜相比，蛋白质在非晶薄膜上的吸附量更大。使用 125I 标记的 HSA 进行的补充研究表明，与椭圆偏振测量相比，蛋白质的吸附量明显增加了六倍。此外，将这四种类型的薄膜在血浆中孵育，然后暴露于抗纤维蛋白原、抗高分子量激肽或抗 C3c，以揭示材料对补体和接触激活的反应。与钛参考材料相比，非晶态和石墨态碳氮化物表现出相当高的免疫活性，而非晶态碳和类富勒烯 CNx 则显示出较少的免疫补体沉积。与参考材料相比，所有薄膜都表现出更强的启动内在凝血途径的能力。最后，通过检查它们在模拟体液中形成磷酸钙晶体的能力，研究了表面的骨结合能力，其中 a-CNx 在孵育 21 天后沉积了最多的磷酸钙。
