Ellerton H D, Blazey N D, Robinson H A
Biochim Biophys Acta. 1977 Nov 25;495(1):140-50. doi: 10.1016/0005-2795(77)90248-3.
Potentiometric and spectrophotometric titrations, isoelectric focusing and amino acid analyses, have been made on the hemocyanin from Jasus edwardsii. Counts of acidic neutral and alkaline groups were made from the titrations, enabling comparisons to be made with the amino acid analysis. Thermodynamic analysis of the data indicated that changes in the native protein structure took place at pH 4.0, 8.4 and 10.7. These observations are discussed in terms of dissociation, shifts in pK and conformational changes in the protein.
已对爱德华岩龙虾血蓝蛋白进行了电位滴定和分光光度滴定、等电聚焦和氨基酸分析。通过滴定确定了酸性、中性和碱性基团的数量，从而能够与氨基酸分析结果进行比较。对数据的热力学分析表明，天然蛋白质结构在pH 4.0、8.4和10.7时发生了变化。从蛋白质的解离、pK值的变化和构象变化方面对这些观察结果进行了讨论。
