Mansfield H W, Ansorge S
Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.
Allerg Immunol (Leipz). 1990;36(4):367-73.
Mitogenesis in peripheral blood lymphocyte and separated T and non-T cells cultures activated with formalin-fixed and heated staphylococcus aureus strain 520 (STA) was studied. The peak of DNA-synthesis in presence of STA is on day 7. The maximum of proliferation was found for T cell on day 7 and for non-T cells on day 3 after stimulation with STA. We found a strong correlation between the expression of CD25 antigen and the 3H-thymidine incorporation. It is concluded that B and T cell proliferation can be studied without purifying the cells using the different proliferation kinetics in presence of STA.
研究了用福尔马林固定并加热的金黄色葡萄球菌520株（STA）激活的外周血淋巴细胞以及分离的T细胞和非T细胞培养物中的有丝分裂。在STA存在下，DNA合成的峰值出现在第7天。用STA刺激后，T细胞在第7天增殖达到最大值，非T细胞在第3天达到最大值。我们发现CD25抗原的表达与3H-胸腺嘧啶核苷掺入之间存在很强的相关性。得出的结论是，在STA存在的情况下，利用不同的增殖动力学，无需纯化细胞就可以研究B细胞和T细胞的增殖。
