免疫化学和质谱分析有和没有选定的糖基化抑制剂的 AGE-BSA 体系中 Nε-(羧甲基)赖氨酸含量。
Immunochemical and mass spectrometric analysis of Nε-(carboxymethyl)lysine content of AGE-BSA systems prepared with and without selected antiglycation agents.
机构信息
Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University, Belfast, Northern Ireland BT9 5AG.
出版信息
J Agric Food Chem. 2010 Nov 24;58(22):11955-61. doi: 10.1021/jf102543g. Epub 2010 Oct 27.
The present study was designed to compare surface plasmon resonance (SPR) biosensor, enzyme-linked immunosorbent assay (ELISA), and ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) methods for the analysis of Nε-(carboxymethyl)lysine (CML) in glucose-bovine serum albumin (BSA) model systems and to investigate the possible inhibitory effect of selected compounds (α-tocopherol, ferulic acid, rutin, thiamin, thiamin monophosphate, and thiamin pyrophosphate) on CML formation. The reported levels of CML detected were dependent upon the method of analysis employed. The highest reported concentrations were obtained with the SPR biosensor, whereas the lowest were found by ELISA. However, a high correlation was observed between these two immunochemical procedures. CML concentrations were dependent upon the type and concentration of the candidate CML inhibitor. All inhibitory compounds investigated, with the exception of α-tocopherol, decreased the level of CML formation in the glucose-BSA system.
本研究旨在比较表面等离子体共振（SPR）生物传感器、酶联免疫吸附测定（ELISA）和超高效液相色谱-串联质谱（UPLC-MS/MS）方法，以分析葡萄糖-牛血清白蛋白（BSA）模型系统中的 Nε-（羧甲基）赖氨酸（CML），并研究选定化合物（α-生育酚、阿魏酸、芦丁、硫胺素、硫胺素单磷酸酯和硫胺素焦磷酸酯）对 CML 形成的可能抑制作用。报告的 CML 水平取决于所采用的分析方法。SPR 生物传感器获得的报告浓度最高，而 ELISA 获得的报告浓度最低。然而，这两种免疫化学程序之间观察到高度相关性。CML 浓度取决于候选 CML 抑制剂的类型和浓度。除α-生育酚外，所有研究的抑制化合物均降低了葡萄糖-BSA 系统中 CML 形成的水平。
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