[特发性肺纤维化和结节病患者血清及支气管肺泡灌洗液中基质金属蛋白酶-1和基质金属蛋白酶-7的变化]

[Change of matrix metalloproteinase-1 and matrix metalloproteinase-7 in serum and bronchoalveolar lavage fluid of patients with idiopathic pulmonary fibrosis and sarcoidosis].

作者信息

Zhou Le-fei, Jiang Li, Li Zhen-hua, Kang Jian

机构信息

Institute of Respiratory Disease, First Affiliated Hospital, China Medical University, Shenyang 110001, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2010 Jun;33(6):441-4.

PMID:20979817

Abstract

OBJECTIVE

To detect the levels of matrix metalloprotease (MMP)-1 and MMP-7 in the serum and the bronchoalveolar lavage fluid (BALF) of patients with idiopathic pulmonary fibrosis (IPF) and sarcoidosis (Stage II), and therefore to investigate the significance of these changes in the pathogenesis of IPF.

METHODS

Forty-four clinically confirmed cases of IPF were recruited, with the patients' age ranging from 46 to 70 years (58 ± 9 years). Twenty patients with sarcoidosis, aged 35 to 65 (50 ± 12) years, were also studied. Enzyme-linked immunoabsorbent assay was used to detect the levels of MMP-1 and MMP-7 in the serum and the BALF samples.

RESULTS

In the serum of patients with IPF, the level of MMP-1 [3.78 (0.14 - 13.44) µg/L] was lower than that in patients with sarcoidosis [7.79 (4.67 - 10.68) µg/L (z = -3.53, P < 0.01)], but the level of MMP-7 [7.83 (3.57 - 14.37) µg/L] was higher than that in patients with sarcoidosis [4.04 (0.06 - 9.94) µg/L (z = -3.84, P < 0.01)]. In the BALF of patients with IPF, the level of MMP-1 [1.09 (0.04 - 5.14) µg/L] was lower than that in patients with sarcoidosis [2.08 (0.05 - 4.16) µg/L (z = -1.53, P > 0.05)], but the level of MMP-7 [3.75 (1.10 - 9.87)µg/L] was higher than that in patients with sarcoidosis [1.16 (0.02 - 4.47) µg/L (z = -5.33, P < 0.01)]. The serum level of MMP-7 in patients with IPF was negatively correlated with the diffusing capacity of carbon monoxide(r = -0.56, P < 0.01) and the percentage of neutrophils (r = -0.47, P < 0.01). The level of MMP-7 in the BALF showed a negative correlation with diffusing capacity of carbon monoxide (r = -0.31, P < 0.05).

CONCLUSIONS

The results suggest that MMP-1 may be increased in the inflammatory phase as compared to the matrix remodeling phase of lung fibrosis, while MMP-7 may be increased in the matrix remodeling phase rather than in the inflammatory phase. MMP-7 may act as an important indicator for the severity of IPF.

摘要

目的

检测特发性肺纤维化（IPF）和结节病（Ⅱ期）患者血清及支气管肺泡灌洗液（BALF）中基质金属蛋白酶（MMP）-1和MMP-7的水平，从而探讨这些变化在IPF发病机制中的意义。

方法

招募44例临床确诊的IPF患者，患者年龄46至70岁（58±9岁）。还研究了20例年龄在35至65岁（50±12岁）的结节病患者。采用酶联免疫吸附测定法检测血清和BALF样本中MMP-1和MMP-7的水平。

结果

IPF患者血清中MMP-1水平[3.78（0.14 - 13.44）μg/L]低于结节病患者[7.79（4.67 - 10.68）μg/L（z = -3.53，P < 0.01）]，但MMP-7水平[7.83（3.57 - 14.37）μg/L]高于结节病患者[4.04（0.06 - 9.94）μg/L（z = -3.84，P < 0.01）]。IPF患者BALF中MMP-1水平[1.09（0.04 - 5.14）μg/L]低于结节病患者[2.08（0.05 - 4.16）μg/L（z = -1.53，P > 0.05）]，但MMP-7水平[3.75（1.10 - 9.87）μg/L]高于结节病患者[1.16（0.02 - 4.47）μg/L（z = -5.33，P < 0.01）]。IPF患者血清MMP-7水平与一氧化碳弥散量呈负相关（r = -0.56，P < 0.01），与中性粒细胞百分比呈负相关（r = -0.47，P < 0.01）。BALF中MMP-7水平与一氧化碳弥散量呈负相关（r = -0.31，P < 0.05）。