Sun De-guang, Wu Yi-feng, Gao Zhen-ming, Liang Rui, Wang Li-ming
Department of General Surgery, Second Affiliated Hospital, Dalian Medical University, China.
Zhonghua Yi Xue Za Zhi. 2010 Jun 22;90(24):1660-4.
To investigate the expression of apoptosis arrestin PED/PEA-15 and XIAP in hepatocellular carcinoma (HCC) and explore their relationship with clinicopathological factors of HCC.
The mRNA expressions of PED/PEA-15 and XIAP genes were detected by RT-PCR (reverse transcription-polymerase chain reaction) in resected liver tumor tissues and corresponding adjacent noncancerous tissues from 40 HCC patients and normal liver tissues from 12 patients with benign lesions. Their protein levels were detected by Western blot.
The mRNA expressions of PED/PEA-15 and XIAP genes and their proteins were significantly higher than those in adjacent tissues and normal tissues (0.636 +/- 0.061, 0.352 +/- 0.068, 0.179 +/- 0.036; 0.579 +/- 0.090, 0.344 +/- 0.084, 0.184 +/- 0.038) (P < 0.01). The mRNA levels of PED/PEA-15 and XIAP genes and their protein levels were significantly associated with the pathological grade and clinical stage of HCC (P < 0.05). However there was not a significant correlation with such clinicopathological features as age, gender, size of tumor, tumor load, metastasis and recurrence (P > 0.05).
The expressions of apoptosis arrestin PED/PEA-15 and XIAP are elevated in HCC as compared with adjacent tissues and normal tissues. Thus it may serve as both a reference indicator for biological behaviors of HCC and a new target for gene therapy.
研究凋亡抑制蛋白PED/PEA-15和XIAP在肝细胞癌（HCC）中的表达情况，并探讨它们与HCC临床病理因素的关系。
采用逆转录聚合酶链反应（RT-PCR）检测40例HCC患者手术切除的肝肿瘤组织及相应癌旁非癌组织，以及12例良性病变患者正常肝组织中PED/PEA-15和XIAP基因的mRNA表达。采用蛋白质免疫印迹法检测其蛋白水平。
PED/PEA-15和XIAP基因的mRNA表达及其蛋白水平均显著高于癌旁组织和正常组织（0.636±0.061，0.352±0.068，0.179±0.036；0.579±0.090，0.344±0.084，0.184±0.038）（P<0.01）。PED/PEA-15和XIAP基因的mRNA水平及其蛋白水平与HCC的病理分级和临床分期显著相关（P<0.05）。然而，与年龄、性别、肿瘤大小、肿瘤负荷、转移和复发等临床病理特征无显著相关性（P>0.05）。
与癌旁组织和正常组织相比，凋亡抑制蛋白PED/PEA-15和XIAP在HCC中表达升高。因此，其可作为HCC生物学行为的参考指标和基因治疗的新靶点。
