Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, PR China.
Bioresour Technol. 2011 Feb;102(3):3020-7. doi: 10.1016/j.biortech.2010.09.122. Epub 2010 Oct 8.
In this study, a systemic analysis was initially performed to investigate the relationship between fermentation-related stress tolerances and ethanol yield. Based on the results obtained, two elite Saccharomyces cerevisiae strains, Z8 and Z15, with variant phenotypes were chosen to construct strains with improved multi-stress tolerance by genome shuffling in combination with optimized initial selection. After three rounds of genome shuffling, a shuffled strain, YZ1, which surpasses its parent strains in osmotic, heat, and acid tolerances, was obtained. Ethanol yields of YZ1 were 3.11%, 10.31%, and 10.55% higher than those of its parent strains under regular, increased heat, and high gravity fermentation conditions, respectively. YZ1 was applied to bioethanol production at an industrial scale. Results demonstrated that the variant phenotypes from available yeast strains could be used as parent stock for yeast breeding and that the genome shuffling approach is sufficiently powerful in combining suitable phenotypes in a single strain.
在这项研究中，我们首先进行了系统分析，以研究与发酵相关的应激耐受能力与乙醇产量之间的关系。基于所获得的结果，选择了两株具有不同表型的优秀酿酒酵母（Saccharomyces cerevisiae）菌株 Z8 和 Z15，通过基因组重排结合优化的初始选择，构建具有改良的多应激耐受性的菌株。经过三轮基因组重排，获得了一株在耐渗、耐热和耐酸方面均优于亲本菌株的重排菌株 YZ1。与亲本菌株相比，YZ1 在常规、高温和高浓度发酵条件下的乙醇产量分别提高了 3.11%、10.31%和 10.55%。YZ1 已应用于工业规模的生物乙醇生产。结果表明，可用酵母菌株的变异表型可作为酵母选育的亲本株，而基因组重排方法在将合适的表型组合到单一菌株中具有足够的威力。
