Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, La Trobe Research and Development Park, Bundoora, VIC 3083, Australia.
Theor Appl Genet. 2011 Feb;122(3):609-22. doi: 10.1007/s00122-010-1473-8. Epub 2010 Oct 28.
Perennial ryegrass is a globally cultivated obligate outbreeding diploid species (2n = 2x = 14) which is subjected to periods of waterlogging stress due to flood irrigation during winter and the lead-up to summer. Reduction of oxygen supply to root systems due to waterlogging produces consequent deleterious effects on plant performance. Framework genetic maps for a large-scale genetic mapping family [F₁(NA(x) × AU₆)] were constructed containing 91 simple sequence repeat and 24 single nucleotide polymorphism genetic markers. Genetic trait dissection using both control and waterlogging treatments was performed in the glasshouse, a total of 143 maximally recombinant genotypes being selected from the overall sib-ship and replicated threefold in the trial. Analysis was performed for nine quantitative morphological traits measured 8 weeks after stress treatments were applied. A total of 37 quantitative trait loci (QTLs) were identified; 19 on the NA(x) parental genetic map, and 18 on the AU₆ parental genetic map. Regions of particular interest were identified on linkage groups (LGs) 4 and 3 of the respective maps, which have been targeted for further analysis by selection of critical recombinants. This first study of genetic control of waterlogging tolerance in ryegrasses has important implications for breeding improvement of abiotic stress adaptation.
黑麦草是一种全球广泛种植的强制异交二倍体物种（2n = 2x = 14），由于冬季和夏季前的洪水灌溉，它会经历水淹胁迫期。由于水淹导致根系供氧减少，会对植物的性能产生相应的有害影响。构建了一个大规模遗传图谱家族[F₁(NA(x) × AU₆)]的框架遗传图谱，其中包含 91 个简单序列重复和 24 个单核苷酸多态性遗传标记。在温室中进行了对照和水淹处理的遗传性状剖析，从总体亲缘关系中选择了 143 个最大重组基因型，并在试验中重复了三倍。在施加胁迫处理 8 周后，对 9 个定量形态性状进行了分析。共鉴定到 37 个数量性状位点（QTL）；NA(x) 亲本遗传图谱上有 19 个，AU₆ 亲本遗传图谱上有 18 个。在各自图谱的连锁群（LG）4 和 3 上确定了特别感兴趣的区域，已通过选择关键重组体来进一步分析这些区域。这项对黑麦草耐水淹遗传控制的首次研究对生物胁迫适应性的育种改良具有重要意义。
