Mark-Malchoff D, Marinetti G V, Hare G D, Meisler A
Biochemistry. 1978 Jun 27;17(13):2684-8. doi: 10.1021/bi00606a035.
A threonine phospholipid in polyoma virus-transformed hamster embryo fibroblasts has been characterized as phosphatidylthreonine. The identification has been made by chemical and enzymatic hydrolysis. Acid hydrolysis of the phospholipid produces free threonine. Mild alcoholysis produces a water-soluble derivative having the properties of glycerophosphorylthreonine. Hydrolysis with phospholipase C produces phosphorylthreonine which on prolonged acid hydrolysis yields threonine. Phosphatidylthreonine in the cell is more accessible to reaction with fluorodinitrobenzene than is phosphatidylserine. Phosphatidylthreonine also has been found as a major aminophospholipid in two other polyoma-transformed hamster cell lines and in the BHK-21/C13 line including the PVT-3 and TS-3 lines. the latter derived from BHK cells. Only a trace amount of phosphatidylthreonine occurs in normal liver, kidney and spleen of the adult mouse, in normal liver and kidney of the adult hamster, in whole mouse and hamster embryos, and in mouse 3T3 cells and SV40-transformed 3T3 cells.
多瘤病毒转化的仓鼠胚胎成纤维细胞中的一种苏氨酸磷脂已被鉴定为磷脂酰苏氨酸。该鉴定是通过化学和酶促水解完成的。磷脂的酸水解产生游离苏氨酸。温和的醇解产生具有甘油磷酰苏氨酸性质的水溶性衍生物。用磷脂酶C水解产生磷酰苏氨酸，长时间酸水解后产生苏氨酸。细胞中的磷脂酰苏氨酸比磷脂酰丝氨酸更容易与氟二硝基苯发生反应。在另外两种多瘤病毒转化的仓鼠细胞系以及包括PVT - 3和TS - 3系在内的BHK - 21/C13系中，磷脂酰苏氨酸也被发现是主要的氨基磷脂，后者源自BHK细胞。在成年小鼠的正常肝脏、肾脏和脾脏、成年仓鼠的正常肝脏和肾脏、整个小鼠和仓鼠胚胎以及小鼠3T3细胞和SV40转化的3T3细胞中，仅存在微量的磷脂酰苏氨酸。
