Nozawa A, Kato K, Uchida S
Department of Viral Infection, University of Tokyo, Japan.
Jpn J Exp Med. 1987 Oct;57(5):267-75.
We studied the surface antigens on hamster cells transformed by SV40, BK and JC viruses using a complement-dependent cytotoxicity test. Antiserum was obtained from guinea pigs immunized with an SV40-transformed hamster cell line, and used after appropriate absorption with a range of cell types. The cell lines transformed by these viruses were all found to possess the following surface antigens: 1) a unique set of organ antigens, 2) fetal antigen(s), and 3) an antigen tentatively named primate polyoma virus-transformed hamster cell antigen (PPH antigen-1). These antigens were not detected on other hamster cell lines, including those transformed by mouse polyoma-, adeno-, herpes-, or retro-viruses Neither could they be detected on mouse, rat or human cells transformed by SV40 or BK viruses. They were, thus, probably host-derived antigens specifically depressed or unmasked by the primate polyoma viruses. In addition, all hamster cell lines tested, including BHK21, carried an antigen(s) named hamster cell line antigen, which could not be detected on surfaces of normal cells, in primary or secondary culture.
我们使用补体依赖性细胞毒性试验研究了经SV40、BK和JC病毒转化的仓鼠细胞表面抗原。抗血清取自用SV40转化的仓鼠细胞系免疫的豚鼠，并在与一系列细胞类型进行适当吸收后使用。发现这些病毒转化的细胞系均具有以下表面抗原：1）一组独特的器官抗原，2）胎儿抗原，3）一种暂命名为灵长类多瘤病毒转化仓鼠细胞抗原（PPH抗原-1）的抗原。在其他仓鼠细胞系上未检测到这些抗原，包括那些经小鼠多瘤病毒、腺病毒、疱疹病毒或逆转录病毒转化的细胞系。在经SV40或BK病毒转化的小鼠、大鼠或人类细胞上也未检测到这些抗原。因此，它们可能是灵长类多瘤病毒特异性抑制或暴露的宿主衍生抗原。此外，所有测试的仓鼠细胞系，包括BHK21，都带有一种名为仓鼠细胞系抗原的抗原，在原代或传代培养的正常细胞表面未检测到这种抗原。
