Giul'khandanian G V, Sukhanov V A, Zhdanov R I, Kuznetsov A N
Biofizika. 1978 Jul-Aug;23(4):589-94.
Investigation of the binding of spin-labeled palmitic acid and its esters with bovine serum albumin is presented. Both probes are shown to bind strictly to strongly and loosely binding centers of protein. The quantitative study allows to determine the binding constant and number of protein binding sites. It is found that the total binding constant for esterified derivative is several times as much as that of palmitic acid.
本文介绍了自旋标记棕榈酸及其酯与牛血清白蛋白结合的研究。两种探针均显示出严格结合到蛋白质的强结合中心和弱结合中心。定量研究有助于确定结合常数和蛋白质结合位点的数量。研究发现,酯化衍生物的总结合常数是棕榈酸的几倍。