Bretz W A, Lopatin D E, Loesche W J
School of Dentistry, University of Michigan, Ann Arbor.
Oral Microbiol Immunol. 1990 Oct;5(5):275-9. doi: 10.1111/j.1399-302x.1990.tb00425.x.
Treponema denticola and Bacteroides gingivalis are among the few recognized species found in periodontal pockets that can hydrolyze the synthetic peptide N-benzoyl-DL-arginine-2-naphthylamide (BANA). We determined the presence of these periodontal pathogens in BANA-positive and -negative plaque samples through the use of indirect immunofluorescent antibody techniques. Eighteen of 27 diseased sites gave BANA-positive reactions, and 9 gave BANA-negative reactions. T. denticola was present in 16 of 18 BANA-positive reactions, whereas B. gingivalis was detected in 9 of the 18 BANA-positive reactions. T. denticola was present in 1 and B. gingivalis in 2 of the 9 BANA-negative reactions. Neither organism was detected in the 19 healthy sites that were negative for BANA. All measured differences between BANA-positive and BANA-negative plaques obtained in the same individuals were statistically significant. The accuracy of the BANA test, compared with clinical parameters such as bleeding upon probing and increased probing depth, was about 80%. The accuracy of the test in detecting the presence of T. denticola was 93%, for B. gingivalis, 76% and for T. denticola and/or B. gingivalis, 96%. This study indicated that BANA-positive plaques were associated with the presence of T. denticola and/or B. gingivalis, that T. denticola was found at a greater frequency and levels in BANA-positive plaques than B. gingivalis, and that the presence of these organisms was associated with clinical disease.
齿垢密螺旋体和牙龈拟杆菌是在牙周袋中发现的少数几种能水解合成肽N-苯甲酰-DL-精氨酸-2-萘酰胺（BANA）的已知菌种。我们通过间接免疫荧光抗体技术确定了这些牙周病原体在BANA阳性和阴性菌斑样本中的存在情况。27个患病部位中有18个出现BANA阳性反应，9个出现BANA阴性反应。在18个BANA阳性反应中，有16个存在齿垢密螺旋体，而在18个BANA阳性反应中有9个检测到牙龈拟杆菌。在9个BANA阴性反应中，有1个存在齿垢密螺旋体，2个存在牙龈拟杆菌。在19个BANA阴性的健康部位均未检测到这两种微生物。在同一个体中获得的BANA阳性和阴性菌斑之间的所有测量差异均具有统计学意义。与诸如探诊出血和探诊深度增加等临床参数相比，BANA检测的准确率约为80%。该检测在检测齿垢密螺旋体存在方面的准确率为93%，牙龈拟杆菌为76%，齿垢密螺旋体和/或牙龈拟杆菌为96%。这项研究表明，BANA阳性菌斑与齿垢密螺旋体和/或牙龈拟杆菌的存在有关，在BANA阳性菌斑中发现齿垢密螺旋体的频率和水平高于牙龈拟杆菌，并且这些微生物的存在与临床疾病有关。
