Gharbia S E, Shah H N, Lawson P A, Haapasalo M
London Hospital Medical College, United Kingdom.
Oral Microbiol Immunol. 1990 Dec;5(6):324-7. doi: 10.1111/j.1399-302x.1990.tb00434.x.
Three reference strains of Fusobacterium nucleatum and 32 human oral isolates were compared by a variety of physiological tests, enzyme electrophoretic profiles, SDS-PAGE patterns, DNA base composition and hybridization to test their possible site specificity and frequency of the recently described subspecies of F. nucleatum. Nine of the 11 isolates assigned to F. nucleatum subspecies nucleatum were from diseased sites, whereas isolates from healthy sites were all identified as F. nucleatum subspecies polymorphum or F. nucleatum subspecies fusiforme. Strains of the latter subspecies were the least frequently isolated (2 of 32). These results, although still inconclusive because of the relatively small sample size, nevertheless confirmed the heterogeneity of F. nucleatum and indicate that most human oral isolates from subgingival sites probably belong to F. nucleatum subspecies nucleatum.
通过多种生理学测试、酶电泳图谱、SDS - PAGE模式、DNA碱基组成和杂交，对3株具核梭杆菌参考菌株和32株人类口腔分离株进行了比较，以测试它们可能的位点特异性以及最近描述的具核梭杆菌亚种的出现频率。在被归为具核梭杆菌具核亚种的11株分离株中，有9株来自患病部位，而来自健康部位的分离株均被鉴定为具核梭杆菌多形亚种或具核梭杆菌梭形亚种。后一个亚种的菌株分离频率最低（32株中有2株）。尽管由于样本量相对较小，这些结果仍无定论，但它们证实了具核梭杆菌的异质性，并表明大多数来自龈下部位的人类口腔分离株可能属于具核梭杆菌具核亚种。
