Rodkey L S
Department of Pathology, University of Texas Medical School, Houston 77225-0708.
Appl Theor Electrophor. 1990;1(5):243-7.
Studies of a zwitterionic buffer formulated for cell electrophoresis were done using the McDonnell-Douglas Continuous Flow Electrophoresis System. Standard buffers were analyzed for their stability in the electrical field and the results showed that both buffers tested were inherently unstable. Further, titration studies showed that the standards buffers buffered poorly at the pH employed for electrophoresis. The zwitterionic buffer buffered well at its nominal pH and was shown to be stable in the electrical field. Comparative studies of the buffer with standard cell separation buffers using formalin fixed rabbit and goose red blood cells showed that the zwitterionic buffer gave better resolution of the fixed cells. Studies with viable hybridoma cells showed that buffer Q supported cell viability equal to Hank's Balanced Salt Solution and that hybridoma cells in different stages of the growth cycle demonstrated reproducible differences in electrophoretic mobility.
使用麦克唐纳 - 道格拉斯连续流电泳系统对一种为细胞电泳配制的两性离子缓冲液进行了研究。分析了标准缓冲液在电场中的稳定性，结果表明所测试的两种缓冲液本质上都是不稳定的。此外，滴定研究表明，标准缓冲液在用于电泳的pH值下缓冲能力较差。两性离子缓冲液在其标称pH值下缓冲良好，并且在电场中表现出稳定性。使用福尔马林固定的兔和鹅红细胞，将该缓冲液与标准细胞分离缓冲液进行比较研究，结果表明两性离子缓冲液对固定细胞具有更好的分辨率。对活杂交瘤细胞的研究表明，缓冲液Q支持的细胞活力与汉克平衡盐溶液相当，并且处于不同生长周期阶段的杂交瘤细胞在电泳迁移率上表现出可重复的差异。
