Hannig K, Kowalski M, Klöck G, Zimmermann U, Mang V
Institut für Biotechnologie, Universität Würzburg, Federal Republic of Germany.
Electrophoresis. 1990 Aug;11(8):600-4. doi: 10.1002/elps.1150110803.
A mixture of fixed rabbit, guinea pig and rat erythrocytes, suspended in a relatively conductive solution, was separated by means of continuous free flow electrophoresis (CFFE) under 1 g- and microgram- conditions using a specially designed electrophoretic module. Short duration microgram conditions were realized on board a sounding rocket. Due to the energy input and the associated thermal convection a separation of the three differently charged cell types in distinct peaks was not possible under 1 g-conditions as shown by reference experiments on the ground before launch. In contrast to the poor resolution under 1 g-conditions, clear separation of the cell mixture could be recorded after lift-off of the rocket under microgram-conditions. Repeated measurements demonstrated that the separation profile was completely stable during the entire microgram-phase of about 6 min. Since the CFFE experiment in space was an exact replica of the ground reference experiments, the results demonstrated unambiguously the potential of CFFE for cell separation under microgram-conditions in media of high ionic strength.
将固定的兔、豚鼠和大鼠红细胞混合，悬浮于相对导电的溶液中，使用专门设计的电泳模块，在1g和微重力条件下通过连续自由流动电泳（CFFE）进行分离。短时间的微重力条件是在探空火箭上实现的。由于能量输入和相关的热对流，如发射前在地面进行的参考实验所示，在1g条件下不可能将三种带不同电荷的细胞类型分离成不同的峰。与1g条件下分辨率较差形成对比的是，火箭升空后在微重力条件下可以记录到细胞混合物的清晰分离。重复测量表明，在约6分钟的整个微重力阶段，分离图谱完全稳定。由于太空中的CFFE实验是地面参考实验的精确复制品，结果明确证明了CFFE在高离子强度介质中微重力条件下进行细胞分离的潜力。
