Magnani M, Stocchi V, Serafini G, Bossù M
Ital J Biochem. 1983 Jan-Feb;32(1):28-35.
The kinetic properties of rabbit red blood cell hexokinase in different buffer systems have been studied. At pH 8.0 the reaction velocity (v) is about 30% higher in glycylglycine compared to Tris, Tea, Hepes or ammonium acetate buffers. The enzyme stability, heat-dependence and spectral properties of the enzyme are also affected by the buffer utilized. None of the following kinetic properties of red blood cell hexokinase varies with pH in the range 6.8-8.5: Km of glucose; Km of ATP and Ki of glucose 6-phosphate.
研究了家兔红细胞己糖激酶在不同缓冲体系中的动力学特性。在pH 8.0时，与Tris、Tea、Hepes或醋酸铵缓冲液相比，甘氨酰甘氨酸缓冲液中的反应速度（v）高约30%。所用缓冲液也会影响该酶的稳定性、热依赖性和光谱特性。家兔红细胞己糖激酶的以下动力学特性在6.8 - 8.5的pH范围内均不随pH变化：葡萄糖的Km；ATP的Km和6-磷酸葡萄糖的Ki。
