Kluytmans J A, van der Willigen A H, van Heyst B Y, van der Meyden W I, Stolz E, Wagenvoort J H
Department of Clinical Microbiology and Antimicrobial Therapy, University Hospital Rotterdam Dijkzig, The Netherlands.
Int J STD AIDS. 1990 Jan;1(1):49-52. doi: 10.1177/095646249000100112.
Chlamydiazyme (Abbott), an enzyme-linked immunoassay (EIA), was evaluated using cell culture on Hela 229 cells as the method of reference. Samples were acquired from 611 female and 280 male patients attending the outpatient clinic for sexually transmitted disease at the University Hospital in Rotterdam, The Netherlands. The prevalences of chlamydia culture-positive female and male patients were 7.8% and 14.4% respectively. The overall sensitivity and specificity values of the EIA were respectively 68.1% and 95.8% in the female and 92.1% and 92.0% in the male population. Samples which were culture-negative but EIA-positive were re-examined by a second direct test (IDEA; Boots Celltech). If the samples from 12 females and 11 males which were negative on culture but positive with both direct tests are considered as failures of cell culture, the sensitivity of the EIA in females almost equalled cell culture (74.6% versus 79.9%) and in males was even higher (93.9% versus 77.6%). Serotyping of the cultured strains revealed that all serovars of Chlamydia trachomatis occurring in this study could be detected by the EIA. The EIA offers a relatively simple and rapid test for diagnosis of C. trachomatis infections in high-risk populations.
采用以人宫颈癌细胞系Hela 229细胞进行细胞培养作为参考方法，对衣原体酶免疫测定法（Chlamydiazyme，雅培公司产品）进行了评估。样本取自荷兰鹿特丹大学医院性传播疾病门诊的611名女性和280名男性患者。衣原体培养阳性的女性和男性患者患病率分别为7.8%和14.4%。酶免疫测定法在女性中的总体敏感性和特异性值分别为68.1%和95.8%，在男性中的总体敏感性和特异性值分别为92.1%和92.0%。对培养阴性但酶免疫测定法阳性的样本，通过第二种直接检测方法（IDEA；博姿细胞技术公司）进行重新检测。如果将12名女性和11名男性培养阴性但两种直接检测均为阳性的样本视为细胞培养失败，那么酶免疫测定法在女性中的敏感性几乎与细胞培养相当（74.6%对79.9%），在男性中的敏感性甚至更高（93.9%对77.6%）。对培养菌株进行血清分型显示，本研究中出现的沙眼衣原体所有血清型均可通过酶免疫测定法检测到。酶免疫测定法为高危人群沙眼衣原体感染的诊断提供了一种相对简单、快速的检测方法。
