Gouttefangeas C, Bensussan A, Boumsell L
INSERM U93, Institut de recherche sur les maladies du sang, Hôpital Saint-Louis, Paris, France.
Nouv Rev Fr Hematol (1978). 1990;32(5):337-40.
We show further differences between two clinically related entities, T cell acute lymphoblastic leukemia (T-ALL) and lymphoblastic lymphoma (T-LL), by using several monoclonal antibodies (mAb) reacting either with constant or variable regions of T cell receptors (TcR) alpha beta and gamma delta or with various CD molecules. We analysed a panel of 15 T-ALL and 15 T-LL selected for their cell surface expression of the CD3 molecules. The results indicate that TcR gamma delta is more frequently used than TcR alpha beta in T-ALL (10 out of the 15 patients tested). This is in contrast to the results obtained with T-LL where the vast majority expressed TcR alpha beta (13 out of the 15 patients). These findings suggest that the leukemic cells could have a different origin in these two diseases. In addition analysis of TcR variable regions expressed by the leukemic blasts showed that in most cases they had rearranged functional V delta 1 gene segments (8 out of 11 patients) whereas in a unique case V delta 2 gene segment was used. Taken together these results and those indicating that T-ALL cell coexpress the CD1a,b and c molecules strengthen the possibility that even though these leukemic cells express the CD3-TcR complex at their cell surface their normal counterparts are not found in peripheral blood.
通过使用几种单克隆抗体（mAb），我们进一步展示了两种临床相关实体——T细胞急性淋巴细胞白血病（T-ALL）和淋巴细胞淋巴瘤（T-LL）之间的差异，这些抗体可与T细胞受体（TcR）αβ和γδ的恒定区或可变区反应，或与各种CD分子反应。我们分析了一组15例T-ALL和15例T-LL，这些病例因细胞表面表达CD3分子而被选中。结果表明，在T-ALL中，TcRγδ比TcRαβ更常被使用（在15例测试患者中有10例）。这与T-LL的结果形成对比，在T-LL中绝大多数表达TcRαβ（15例患者中有13例）。这些发现表明，这两种疾病中的白血病细胞可能有不同的起源。此外，对白血病母细胞表达的TcR可变区的分析表明，在大多数情况下，它们重排了功能性Vδ1基因片段（11例患者中有8例），而在一个独特的病例中使用了Vδ2基因片段。综合这些结果以及那些表明T-ALL细胞共表达CD1a、b和c分子的结果，进一步加强了这样一种可能性，即尽管这些白血病细胞在其细胞表面表达CD3-TcR复合物，但在外周血中找不到它们的正常对应物。
