Iordanov I, Baĭnova A, Chipilska L
Probl Khig. 1990;15:30-8.
Studies are performed on the new Bulgarian raw materials "Aminookis" (AO) and "Dezodorirashch agent" (DA) the plants "Alen Mak"--Plovdiv and "Hyaluron"--Sofia. Chemical, toxicological and microbiological studies are carried out. Thin-layer chromatographic and spectrophotometric methods for determination of AO, hyaluronic acid and DA are developed and chemical studies on cosmetics, prepared with them, are carried out. During the repetition of a 21-day experiment on guinea pigs the threshold irritative concentrations--10% for AO and DA (as trade products) are determined. This shows that they are moderate contact irritants. The hyaluron has no irritative effect. By maximizing test with guinea pigs is established the absence of contact allergy for DA and AO. At index of sensibilization 10% the hyaluron falls in the group of weak allergens. The spectra of action are studied and the minimum suppressing concentrations of AO and DA are defined. It is established that the most resistant to effect with these cosmetics are the representatives of the families: Pseudomonas, Klebsiella, Escherichia, Salmonella. Recommendations are made to use "Aminookis" (AO) in shampoos to 8%, deodorizing agent in deodorants to 2% and "Hyaluron" in creams to 20% (as trade products).
对保加利亚的新原料“Aminookis”（AO）和“Dezodorirashch agent”（DA）在普罗夫迪夫的“Alen Mak”工厂和索非亚的“Hyaluron”工厂进行了研究。开展了化学、毒理学和微生物学研究。开发了用于测定AO、透明质酸和DA的薄层色谱法和分光光度法，并对用它们制备的化妆品进行了化学研究。在豚鼠身上重复进行为期21天的实验期间，确定了阈值刺激浓度——AO和DA（作为商品）为10%。这表明它们是中度接触性刺激物。透明质酸没有刺激作用。通过对豚鼠进行最大限度测试，确定DA和AO不存在接触性过敏。在致敏指数为10%时，透明质酸属于弱过敏原组。研究了作用谱并确定了AO和DA的最小抑制浓度。已确定对这些化妆品作用最具抗性的是以下菌属的代表：假单胞菌属、克雷伯菌属、大肠杆菌属、沙门氏菌属。建议在香波中使用“Aminookis”（AO）的比例为8%，在除臭剂中使用除臭剂的比例为2%，在面霜中使用“Hyaluron”的比例为20%（作为商品）。
