Baz M J, Vargas F, Haro J M, Castillo M A, Jódar E, Andrade J L, García del Río C
Departamento de Bioquímica, Facultad de Medicina, Universidad de Granada, España.
Rev Esp Fisiol. 1990 Dec;46(4):385-91.
It has been suggested that sodium renal excretion is regulated, at least partially, by a factor with natriuretic properties called digoxin-like factor (DLF). As this substance crossreacts with digoxin antibodies, it was measured with a radioimmunoassay used to determine exogenous digoxin. Methodological conditions and quality control to determine DLF in plasma and urine have been established. Good correlation coefficients in specificity as well as dilution studies were obtained. Within--and between--assay coefficients of variations indicate good reproducibility. Moreover, changes in plasma DLF levels were detected in patients with cirrhosis or with renal failure, diseases which thrive on alterations in salt and water metabolism. In conclusion, this radioimmunoassay method for measuring DLF may be useful to investigate the role of this factor in several physiological and pathological conditions.
有人提出，肾脏对钠的排泄至少部分受一种具有利钠特性的物质调节，这种物质称为地高辛样因子（DLF）。由于该物质与地高辛抗体发生交叉反应，因此采用用于测定外源性地高辛的放射免疫分析法对其进行测量。已经建立了测定血浆和尿液中DLF的方法条件和质量控制。在特异性以及稀释研究中获得了良好的相关系数。批内和批间变异系数表明具有良好的重现性。此外，在肝硬化或肾衰竭患者中检测到血浆DLF水平的变化，这些疾病依赖于盐和水代谢的改变。总之，这种测量DLF的放射免疫分析方法可能有助于研究该因子在多种生理和病理状况中的作用。
