多溴联苯对大鼠肺泡中腺苷酸环化酶活性的影响。

Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.

作者信息

Sidhu K S, Michelakis A M

出版信息

Environ Health Perspect. 1978 Apr;23:329-31. doi: 10.1289/ehp.7823329.

DOI:10.1289/ehp.7823329

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1637478/

Abstract

The in vitro effect of polybrominated biphenyls (FireMaster BP-6) on adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes of rat lung alveoli was determined. Two fractions of plasma membranes, PM-I (approximate d = 1.13 g/cm3) and PM-II (approximate d = 1.16 g/cm3) were isolated under hypotonic homogenization and fractionation conditions (1mM NaHCO3, 5mM dithiothreitol, pH 7.5) by using differential centrifugation and nonlinear sucrose gradient centrifugation techniques. Polybrominated biphenyls (10 microgram/ml) dissolved in ethyl acetate (0.25%) stimulated basal adenylate cyclase activity of plasma membranes by 2.1 to 2.5-fold. The implication of this observation is discussed.

摘要

测定了多溴联苯（FireMaster BP - 6）对大鼠肺泡质膜中腺苷酸环化酶[ATP焦磷酸裂解酶（环化），EC 4.6.1.1]活性的体外作用。在低渗匀浆和分级分离条件（1mM碳酸氢钠，5mM二硫苏糖醇，pH 7.5）下，采用差速离心和非线性蔗糖梯度离心技术分离出两部分质膜，即PM - I（密度约为1.13 g/cm³）和PM - II（密度约为1.16 g/cm³）。溶解于乙酸乙酯（0.25%）中的多溴联苯（10微克/毫升）可使质膜的基础腺苷酸环化酶活性提高2.1至2.5倍。讨论了该观察结果的意义。

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