Kassis S, Kindler S H
Biochim Biophys Acta. 1975 Jun 24;391(2):513-6. doi: 10.1016/0005-2744(75)90277-6.
The membrane bound adenylate cyclase (ATP pyrophosphate-lyase (cyclizing) EC 4.6.1.1) of the ciliate Tetrahymena pyriformis could be extracted by washing the membrane fraction with 0.25 M sucrose. The enzyme dissociated in this way did not sediment after centrifugation at 105 000 times g for 2 h and was still responsive to stimulation by epinephrine. Dispersion of the membranes with Triton-X 100 led to purified preparation of the cyclase, which was no longer stimulated by epinephrine but retained fully the activation by fluoride and serotonin.
用0.25M蔗糖洗涤梨形四膜虫的膜部分，可以提取出膜结合腺苷酸环化酶（ATP焦磷酸裂解酶（环化），EC 4.6.1.1）。以这种方式解离的酶在105000倍重力下离心2小时后不会沉淀，并且仍然对肾上腺素的刺激有反应。用Triton-X 100分散膜可得到纯化的环化酶制剂，该制剂不再受肾上腺素刺激，但仍完全保留氟化物和血清素的激活作用。
