Biggiogera M, Bürki K, Kaufmann S H, Shaper J H, Gas N, Amalric F, Fakan S
Centre of Electron Microscopy, University of Lausanne, Switzerland.
Development. 1990 Dec;110(4):1263-70. doi: 10.1242/dev.110.4.1263.
The ultrastructural distribution of proteins B23 and nucleolin in the nucleolus of mouse embryos from the zygote to the early blastocyst has been analyzed by means of specific antibodies and immunocytochemistry using colloidal gold complexes as markers. In parallel, silver staining of nucleoli was carried out on ultrathin sections. Our results show that the compact prenucleolar bodies at 1- and 2-cell stage as well as the compact residual fibrillar masses observed up to the morula stage, are labelled with the two antibodies. These masses, however, are not stained with silver up to the 4-cell stage. In well-developed nucleoli, the two antibodies co-localize in the dense fibrillar component (DFC) and the granular component (GC) while fibrillar centers (FCs) are devoid of label. On the contrary, silver staining occurs in the FCs and DFC but not in the GC. Our observations suggest that there is no direct relationship between the occurrence of silver staining and the distribution of protein B23 or nucleolin. Moreover, neither the localization of the two above proteins nor silver staining are unequivocally related to the nucleolar activity.
利用特异性抗体和免疫细胞化学方法，以胶体金复合物作为标记物，分析了从受精卵到早期囊胚阶段小鼠胚胎核仁中蛋白质B23和核仁素的超微结构分布。同时，对超薄切片进行核仁银染。我们的结果表明，1细胞和2细胞阶段的致密前核仁体以及直至桑椹胚阶段观察到的致密残留纤维团，都被这两种抗体标记。然而，这些团块在4细胞阶段之前都未被银染。在发育良好的核仁中，这两种抗体共定位于致密纤维成分（DFC）和颗粒成分（GC），而纤维中心（FCs）没有标记。相反，银染出现在FCs和DFC中，而不在GC中。我们的观察结果表明，银染的出现与蛋白质B23或核仁素的分布之间没有直接关系。此外，上述两种蛋白质的定位以及银染都与核仁活性没有明确的关联。
