Biggiogera M, Fakan S, Kaufmann S H, Black A, Shaper J H, Busch H
Centre of Electron Microscopy, University of Lausanne, Switzerland.
J Histochem Cytochem. 1989 Sep;37(9):1371-4. doi: 10.1177/37.9.2768807.
The intranucleolar distribution of phosphoproteins B23 and C23 was visualized simultaneously by post-embedding immunoelectron microscopy in HeLa cell nucleoli, using specific antibodies. The data show that proteins B23 and C23 co-localize to the same nucleolar compartments, i.e., the dense fibrillar component and the granular component. Neither of the two antibodies is significantly associated with the fibrillar centers in these cells, although the fibrillar centers appear positive after silver staining. These findings suggest that other unidentified components must be responsible for the silver staining observed in the fibrillar centers of interphase nucleoli. The results are discussed in the light of previously reported data obtained by preembedding immunolabeling techniques and by silver staining, which both suggested a localization of protein C23 inside the fibrillar centers.
使用特异性抗体，通过包埋后免疫电子显微镜技术，在HeLa细胞核仁中同时观察到磷蛋白B23和C23的核仁内分布。数据显示，蛋白B23和C23共定位于相同的核仁区室，即致密纤维组分和颗粒组分。尽管在银染后纤维中心呈阳性，但这两种抗体均未与这些细胞中的纤维中心显著相关。这些发现表明，其他未鉴定的成分必定是间期核仁纤维中心观察到的银染的原因。根据先前通过包埋前免疫标记技术和银染获得的数据对结果进行了讨论，这两项技术均表明蛋白C23定位于纤维中心内部。
