Huhtaniemi I, Martikainen H, Tikkala L
Mol Cell Endocrinol. 1978 Jun;11(1):43-50. doi: 10.1016/0303-7207(78)90031-x.
Adult male rats were injected subcutaneously with a mixture of hCG and [125I]hCG (3, 5, 10, 100 or 1000 IU), and killed 3, 6, 16, 24 or 48 h after injection. Specific uptake of [125I]hCG by the testes as well as the remaining in vitro binding capacity were measured. Cycloheximide (1.5 mg/kg) was given together with hCG to another group of animals. The effect of hCG on testicular LH/hCG receptors was found to consist of two phases: first, 6--16 h after injection, a significant increase in the binding capacity occurred, which was found with the lower 3--10 IU doses of hCG. Thereafter there was a pronounced decrease in receptor concentration, which was directly correlated to the dose of hCG. Experiments with cycloheximide suggest that the decrease in binding, but not the increase, is at least partly dependent on protein synthesis.
成年雄性大鼠皮下注射人绒毛膜促性腺激素（hCG）和[125I]hCG的混合物（3、5、10、100或1000国际单位），并在注射后3、6、16、24或48小时处死。测定睾丸对[125I]hCG的特异性摄取以及剩余的体外结合能力。向另一组动物同时给予环己酰亚胺（1.5毫克/千克）和hCG。发现hCG对睾丸促黄体生成素/人绒毛膜促性腺激素（LH/hCG）受体的影响包括两个阶段：第一，注射后6 - 16小时，结合能力显著增加，这在较低剂量（3 - 10国际单位）的hCG作用下即可出现。此后，受体浓度明显降低，且与hCG剂量直接相关。环己酰亚胺实验表明，结合能力的降低（而非增加）至少部分依赖于蛋白质合成。
