通过在聚（苯乙烯 - 二乙烯基苯）共聚物上进行液相色谱法分离金霉素和4-表金霉素的酮 - 烯醇互变异构体。

Separation of keto-enol tautomers of chlortetracycline and 4-epichlortetracycline by liquid chromatography on poly(styrene-divinylbenzene)copolymer.

作者信息

Roets E, Busson R, Hoogmartens J

机构信息

Katholieke Universiteit Leuven, Instituut voor Farmaceutische Wetenschappen, Belgium.

出版信息

J Pharm Biomed Anal. 1990;8(8-12):881-9. doi: 10.1016/0731-7085(90)80137-e.

DOI:10.1016/0731-7085(90)80137-e

PMID:2100637

Abstract

Keto and enol tautomers of chlortetracycline and 4-epichlortetracycline were successfully separated by low temperature high-performance liquid chromatography on poly(styrene-divinylbenzene)copolymer. The keto-enol tautomerism occurs between C-11a and C-12. The elucidation of the chemical structure of the tautomers was realized with the help of on-line and off-line UV-spectrophotometry and of 13C and 1H NMR spectrometry. The kinetics of the equilibrium reaction were investigated.

摘要

通过在聚（苯乙烯 - 二乙烯基苯）共聚物上进行低温高效液相色谱法，成功分离了金霉素和4 - 表金霉素的酮式和烯醇式互变异构体。酮 - 烯醇互变异构发生在C - 11a和C - 12之间。借助在线和离线紫外分光光度法以及13C和1H核磁共振光谱法，实现了互变异构体化学结构的阐明。研究了平衡反应的动力学。