采用聚（苯乙烯 - 二乙烯基苯）填充材料通过高效液相色谱法分离红霉素及其相关物质。

Separation of erythromycin and related substances by high-performance liquid chromatography on poly(styrene-divinylbenzene) packing materials.

作者信息

Kibwage I O, Roets E, Hoogmartens J, Vanderhaeghe H

出版信息

J Chromatogr. 1985 Aug 23;330(2):275-86. doi: 10.1016/s0021-9673(01)81984-1.

DOI:10.1016/s0021-9673(01)81984-1

Abstract

A comparative evaluation of three brands of poly(styrene-divinylbenzene) copolymers, Hamilton PRP-1 (10 micron), Rogel (8 micron) and TSK-Gel (10 micron), as column packing materials for high-performance liquid chromatographic separation of erythromycins is presented. Erythromycins A, B and C, anhydroerythromycin A, erythromycin A enol ether, N-demethylerythromycin A, anhydro N-demethylerythromycin A and N-demethylerythromycin A enol ether were chromatographed. The effects of column temperature, concentration of organic modifier in the mobile phase, concentration of phosphate buffer, the addition of quaternary ammonium salts and pH are described. The best separations were obtained on TSK-Gel with the mobile phase acetonitrile-methanol-0.2 M tetramethylammonium hydroxide pH 8.0-0.2 M phosphate buffer pH 8.0-water (30:15:25:5:25). PRP-1 and Rogel gave equally good separations but with higher retention volumes.

摘要

本文介绍了三种品牌的聚（苯乙烯-二乙烯基苯）共聚物，即Hamilton PRP-1（10微米）、Rogel（8微米）和TSK-Gel（10微米），作为高效液相色谱分离红霉素的柱填充材料的比较评估。对红霉素A、B和C、脱水红霉素A、红霉素A烯醇醚、N-去甲基红霉素A、脱水N-去甲基红霉素A和N-去甲基红霉素A烯醇醚进行了色谱分析。描述了柱温、流动相中有机改性剂浓度、磷酸盐缓冲液浓度、季铵盐的添加和pH值的影响。在TSK-Gel上，以乙腈-甲醇-0.2M氢氧化四甲铵pH 8.0-0.2M磷酸盐缓冲液pH 8.0-水（30:15:25:5:25）为流动相，获得了最佳分离效果。PRP-1和Rogel的分离效果同样良好，但保留体积更高。