Watson D G, Midgley J M, Chen R N, Huang W, Bain G M, McDonald N M, Reid J L, McGhee C N
Department of Pharmacy, University of Strathclyde, Glasgow, UK.
J Pharm Biomed Anal. 1990;8(8-12):899-904. doi: 10.1016/0731-7085(90)80139-g.
GC-NICIMS has been employed in the analysis of biogenic amines and their metabolites in human urine and human, bovine and porcine aqueous and vitreous humour. Several new chemical derivatization procedures have been developed in order to analyse these compounds. Concentrations of octopamines and synephrines were determined in urine from treated and untreated hypertensive subjects and normotensive individuals; there were no significant differences in concentrations of these metabolites between these groups. Human urine contained several dihydroxy-phenylethylamines which have not been reported as natural metabolites before and also 5- and 6-hydroxydopamine in relatively large amounts. Aqueous and vitreous humour contained very low quantities of noradrenaline, tyramine and dopamine but measurements were inconsistent because sometimes the levels were below the limits of detection. Metabolites of a number of biogenic amines were readily detected in aqueous and vitreous humour.
气相色谱-负离子化学电离质谱联用仪（GC-NICIMS）已用于分析人尿以及人、牛和猪的房水和玻璃体液中的生物胺及其代谢物。为了分析这些化合物，已开发了几种新的化学衍生化程序。测定了经治疗和未经治疗的高血压患者以及血压正常个体尿液中的去甲肾上腺素和辛弗林浓度；这些组之间这些代谢物的浓度没有显著差异。人尿中含有几种以前未报道为天然代谢物的二羟基苯乙胺，还含有相对大量的5-和6-羟基多巴胺。房水和玻璃体液中去甲肾上腺素、酪胺和多巴胺的含量非常低，但测量结果不一致，因为有时水平低于检测限。在房水和玻璃体液中很容易检测到多种生物胺的代谢物。
