Watson D G, Zhou P, Midgley J M, Milligan C D, Kaiser K
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
J Pharm Biomed Anal. 1993 Nov-Dec;11(11-12):1145-9. doi: 10.1016/0731-7085(93)80096-j.
A range of biogenic amines were measured in the heads from four strains of Drosophila melanogaster. Quantitation was carried out using gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) with stable isotope dilution. The principal amines detected in the heads were dopamine, noradrenaline and 5 HT with small amounts of p- and m-tyramine; p-octopamine could not be detected in samples of 25 heads with a limit of detection of 10 pg per sample. In addition to conventional neurotransmitters or putative neurotransmitters the amines 5- and 6-hydroxydopamine were detected in the heads in substantial amounts.
对四种黑腹果蝇品系头部的一系列生物胺进行了测定。使用气相色谱 - 负离子化学电离质谱法（GC - NICIMS）并结合稳定同位素稀释法进行定量分析。在头部检测到的主要胺类物质是多巴胺、去甲肾上腺素和5 - 羟色胺，同时还有少量的对 - 酪胺和间 - 酪胺；在25个头部样本中未检测到对 - 章鱼胺，每个样本的检测限为10皮克。除了传统的神经递质或假定的神经递质外，在头部还大量检测到了5 - 羟基多巴胺和6 - 羟基多巴胺。
