Rus V, Malide D, Boloşiu H D, Parasca I, Duţu A L
Department of Rheumatology, Medical Clinic No. 2, Cluj-Napoca, Romania.
Med Interne. 1990 Oct-Dec;28(4):305-10.
Activation of the terminal complement pathway leads to formation of the C5b--9 complex. The main effects of C5b--9 generation are tissue injury by cell lysis or by stimulation of proinflammatory mediators. In a study carried out in 42 patients, using polyclonal antibodies against C5b--9 neoantigens and C9 in an ELISA assay, we found significantly higher levels of SC5b--9 complex in plasma from the 18 patients with active systemic lupus erythematosus than those found in 10 healthy controls (p less than 0.005). In the 18 patients presenting rheumatoid arthritis and the 6 with progressive systemic sclerosis the plasma levels of SC5b--9 complex did not differ significantly from those in controls. The SC5b--9 levels found in the synovial fluid samples from the 16 rheumatoid arthritis patients were higher than the corresponding plasma ones. The ratio between synovial fluid and plasma levels was 1.2. Immunoperoxidase staining for C5b--9 was intense in three rheumatoid synovial membranes and absent in two normal synovial membranes obtained during meniscectomy. Increased levels of plasma and synovial fluid SC5b--9 reflect pathologic systemic or local activation of the complement carcase in systemic lupus erythematosus and respectively rheumatoid arthritis. Synovial membrane deposits of C5b--9 are indicative for the lytic and proinflammatory effects of complement activation.
终末补体途径的激活导致C5b-9复合物的形成。C5b-9生成的主要作用是通过细胞溶解或刺激促炎介质引起组织损伤。在一项对42例患者进行的研究中，使用酶联免疫吸附测定法（ELISA）检测针对C5b-9新抗原和C9的多克隆抗体，我们发现18例活动性系统性红斑狼疮患者血浆中的SC5b-9复合物水平显著高于10例健康对照者（p<0.005）。18例类风湿关节炎患者和6例进行性系统性硬化症患者血浆中SC5b-9复合物水平与对照者无显著差异。16例类风湿关节炎患者滑膜液样本中的SC5b-9水平高于相应的血浆水平。滑膜液与血浆水平之比为1.2。在3例类风湿滑膜组织中，C5b-9的免疫过氧化物酶染色呈强阳性，而在半月板切除术时获取的2例正常滑膜组织中未检测到。血浆和滑膜液中SC5b-9水平升高分别反映了系统性红斑狼疮和类风湿关节炎中补体系统的病理性全身或局部激活。滑膜组织中C5b-9的沉积表明补体激活具有溶解和促炎作用。
