[酶联免疫吸附测定法检测血浆中SC5b-9水平及其在系统性红斑狼疮疾病中的临床意义]

[ELISA for detection of SC5b-9 levels in plasma and its clinical significance in SLE disease].

作者信息

Kan D

机构信息

Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1993 Aug;15(4):302-5.

PMID:8168211

Abstract

Using IgG from rabbit antiserum to human MAC neoantigens and SC5b-9, we established a sandwich ELISA for detecting SC5b-9 levels in human plasma. SC5b-9 was measured in 51 normal plasma samples and 40 plasma samples from patients with SLE. We found that SC5b-9 levels in 96.1% of the controls and in 78.9% of the patients with inactive SLE were below 450 ng/ml, while SC5b-9 levels in 81.0% of the patients with active SLE were above 450 ng/ml. This shows that SC5b-9 levels may be useful for diagnosing patients with clinically active SLE.

摘要

我们使用兔抗人膜攻击复合物（MAC）新抗原和SC5b - 9的抗血清中的IgG，建立了一种夹心酶联免疫吸附测定法（ELISA），用于检测人血浆中的SC5b - 9水平。对51份正常血浆样本和40份系统性红斑狼疮（SLE）患者的血浆样本进行了SC5b - 9检测。我们发现，96.1%的对照组和78.9%的非活动期SLE患者的SC5b - 9水平低于450 ng/ml，而81.0%的活动期SLE患者的SC5b - 9水平高于450 ng/ml。这表明SC5b - 9水平可能有助于诊断临床活动期SLE患者。