Souza M E, Castro-e-Silva Júnior O, Picinato M A, Franco C F, Mazzetto S A, Ceneviva R, Roselino J E
Disciplina de Gastroenterologia Cirúrgica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1990;23(10):995-7.
Serum alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) levels are normal or discretely increased in rats with chronic extrahepatic cholestasis (CEHC). During the acute phase (first 72 h after biliary obstruction), however, serum transaminase values are quite elevated due to a mechanism not yet fully elucidated. Thus, this is a good experimental model, not involving hepatocellular necrosis, for the study of serum ALT and AST levels during the acute phase of CEHC. Male Wistar rats (250-350 g) were divided into two groups: group A (N = 60) was submitted to sham operation for bile duct ligation (BDL), and group B (N = 60) was submitted to BDL. Thirty and 120 min after BDL there was a 1.5-fold increase in both serum ALT and AST levels compared to sham-operated rats (P less than 0.05). Serum ALT levels were higher than AST levels as early as 30 min after BDL and the highest serum values for both transaminases were observed at 360 min which was also the last value measured. Serum AST levels increased 120 min after BDL, with no further significant increases thereafter.
在患有慢性肝外胆汁淤积（CEHC）的大鼠中，血清丙氨酸氨基转移酶（ALT）和血清天冬氨酸氨基转移酶（AST）水平正常或轻度升高。然而，在急性期（胆管阻塞后的最初72小时），由于一种尚未完全阐明的机制，血清转氨酶值会显著升高。因此，这是一个不涉及肝细胞坏死的良好实验模型，用于研究CEHC急性期的血清ALT和AST水平。雄性Wistar大鼠（250 - 350克）分为两组：A组（N = 60）进行假胆管结扎手术，B组（N = 60）进行胆管结扎手术。胆管结扎后30分钟和120分钟，与假手术大鼠相比，血清ALT和AST水平均升高了1.5倍（P < 0.05）。胆管结扎后30分钟血清ALT水平就高于AST水平，两种转氨酶的血清最高值在360分钟时出现，这也是测量的最后一个值。血清AST水平在胆管结扎后120分钟升高，此后无进一步显著升高。
