缺血后短期低温对沙鼠脑的保护作用。

Protective effect of short-term post-ischemic hypothermia on the gerbil brain.

Coimbra C G, Cavalheiro E A

Departamento de Neurologia e Neurocirurgia, Escola Paulista de Medicina, São Paulo, Brasil.

Braz J Med Biol Res. 1990;23(6-7):605-11.

Abstract

The effects of post-ischemic hypothermia were studied in the gerbil brain. After 5 min of bilateral common carotid occlusion (BCCO) under thiopental anesthesia and normothermia (rectal temperature of 37 +/- 0.5 degrees C) 20 animals were maintained either normothermic (group NT, N = 10) or hypothermic (rectal temperature of 29 +/- 0.5 degrees C, obtained within 5 min of carotid recirculation) for 5 h (group HT, N = 10). Sham-operated animals (N = 5) were kept normothermic for 5 h following the surgical procedure. 2. After a 7-day period of survival, damage to the dorsal hippocampus was determined histopathologically by cresyl-violet staining and graded on a scale of 0 to 3. The histopathological damage observed in the CA1 subfield of the hippocampus was found to be more intense in NT than in HT gerbils (P less than 0.001, Mann-Whitney U-test). 3. These results suggest that moderate and short-lasting hypothermia induced early in the recirculation period protects the brain against ischemic injury. The importance of these results is discussed in terms of pathophysiology, treatment and interpretation of experimental brain ischemia data.

摘要

在沙鼠脑内研究了缺血后低温的作用。在硫喷妥钠麻醉和正常体温（直肠温度为37±0.5℃）下进行5分钟双侧颈总动脉闭塞（BCCO）后，20只动物被维持在正常体温（NT组，N = 10）或低温状态（直肠温度为29±0.5℃，在颈动脉再灌注后5分钟内达到）5小时（HT组，N = 10）。假手术动物（N = 5）在手术操作后维持正常体温5小时。2. 在存活7天后，通过甲酚紫染色对背侧海马的损伤进行组织病理学测定，并按0至3级进行分级。发现在海马CA1亚区观察到的组织病理学损伤在NT组沙鼠中比HT组沙鼠更严重（P < 0.001，曼-惠特尼U检验）。3. 这些结果表明，在再灌注期早期诱导的中度和短期低温可保护脑免受缺血性损伤。从病理生理学、治疗以及实验性脑缺血数据的解释等方面讨论了这些结果的重要性。