Dizdaroglu M, Hermes W, Schulte-Frohlinde D, von Sonntag C
Int J Radiat Biol Relat Stud Phys Chem Med. 1978 Jun;33(6):563-9. doi: 10.1080/09553007814550471.
Aqueous solutions of DNA were gamma-irradiated in the presence and absence of oxygen and enzymatically hydrolysed by the combined action of pancreatic deoxyribonuclease (DNase I), snake-venom phosphodiesterase (PDE I), spleen phosphodiesterase (PDE II) and alkaline phosphatase. In contrast to unirradiated DNA, which is fully hydrolysed to nucleosides by these enzymes, gamma-irradiated DNA yields a series of oligonucleotides. Their isolation might enalbe the future identification of the chemical nature of DNA lesions.
DNA的水溶液在有氧和无氧条件下进行γ射线辐照，然后通过胰脱氧核糖核酸酶（DNase I）、蛇毒磷酸二酯酶（PDE I）、脾磷酸二酯酶（PDE II）和碱性磷酸酶的联合作用进行酶解。与未辐照的DNA不同，未辐照的DNA可被这些酶完全水解为核苷，而γ射线辐照的DNA会产生一系列寡核苷酸。它们的分离可能有助于未来鉴定DNA损伤的化学性质。
