β-羟基丁酸对胰岛素与其受体结合及受体自身磷酸化的影响。

Effects of beta-hydroxy butyric acid on insulin binding to its receptor and on autophosphorylation of the receptor.

作者信息

Ohtsuka H, Yamauchi K, Ohara N, Miyamoto T, Ichikawa K, Hashizume K

机构信息

Department of Geriatrics, Endocrinology and Metabolism, Shinshu University School of Medicine, Matsumoto, Japan.

出版信息

Endocrinol Jpn. 1990 Dec;37(6):915-922. doi: 10.1507/endocrj1954.37.915.

DOI:10.1507/endocrj1954.37.915

PMID:2101795

Abstract

We examined the effects of beta-hydroxy butyric acid (B-OH-butyrate) on insulin binding to its receptor and on autophosphorylation of the receptor in vitro. The affinity of isolated rat adipocyte insulin receptors was significantly increased by B-OH-butyrate at neutral pH. Similar results were obtained with soluble insulin receptors prepared from hepatocytes. Autophosphorylation of insulin receptor, however, was not affected by OH-butyrate at neutral pH. These results suggested insulin resistance in ketoacidosis is not caused by an interaction between B-OH-butyrate and the insulin receptor.

摘要

我们在体外研究了β-羟基丁酸（B-OH-丁酸酯）对胰岛素与其受体结合以及受体自身磷酸化的影响。在中性pH条件下，B-OH-丁酸酯可显著提高分离的大鼠脂肪细胞胰岛素受体的亲和力。从肝细胞制备的可溶性胰岛素受体也得到了类似结果。然而，在中性pH条件下，胰岛素受体的自身磷酸化不受OH-丁酸酯的影响。这些结果表明，酮症酸中毒时的胰岛素抵抗并非由B-OH-丁酸酯与胰岛素受体之间的相互作用引起。